FUNCTIONAL DIFFERENCES BETWEEN MAMMALIAN NUCLEAR-PROTEIN MATRICES AND PORE-LAMINA COMPLEX LAMINAE

Nuclear matrix isolated from rat liver in the presence of a serine protease inhibitor contained 85% of the rapidly-labelled hnRNA, but pore-lamina preparations from the same tissue contained virtually none of the rapidly-labelled material. Nuclear matrix from rat endometrium, but not from lung, contained high-affinity binding sites for 17β-estradiol, but very few such sites were detected in endometrium pore-lamina preparations. It is concluded that the peripheral (porelamina) and intranuclear parts of the matrix differ functionally. © 1979.