HIGH-SPEED AUTOMATED DNA SEQUENCING IN ULTRATHIN SLAB GELS

被引：73

作者：

KOSTICHKA, AJ

MARCHBANKS, ML

BRUMLEY, RL

DROSSMAN, H

SMITH, LM

机构：

[1] Department of Chemistry, University of Wisconsin-Madison, Madison, WI

来源：

BIO-TECHNOLOGY | 1992年 / 10卷 / 01期

关键词：

D O I：

10.1038/nbt0192-78

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have developed a high speed instrument for automated DNA sequence analysis. The apparatus-employs laser excitation and a cooled CCD detector for the parallel detection of up to 18 sets of four fluorescently labeled DNA sequencing reactions during their electrophoretic separation in ultrathin (50-100-mu-m) denaturing polyacrylamide gels. Four hundred and fifty bases of sequence information is obtained from 100 ng of M13 template DNA in less than one hour, corresponding to an overall instrument throughput of over 8000 bases/hr.

引用

页码：78 / 81

页数：4

共 24 条

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