THE PRECURSOR FORM OF THE RAT-LIVER NONSPECIFIC LIPID-TRANSFER PROTEIN, EXPRESSED IN ESCHERICHIA-COLI, HAS LIPID TRANSFER ACTIVITY

被引：16

作者：

OSSENDORP, BC

GEIJTENBEEK, TBH

WIRTZ, KWA

机构：

[1] Centre for Biomembranes and Lipid Enzymology, State University of Utrecht, 3584 CH Utrecht

来源：

FEBS LETTERS | 1992年 / 296卷 / 02期

关键词：

NONSPECIFIC LIPID-TRANSFER PROTEIN; STEROL CARRIER PROTEIN-2; LIPID TRANSFER; BACTERIAL EXPRESSION; RAT LIVER;

D O I：

10.1016/0014-5793(92)80374-P

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The cDNA encoding the precursor form of non-specific lipid-transfer protein (pre-nsL-TP) from rat liver was cloned into the expression vector pET3d. The resulting plasmid was transformed to the Escherichia coli strain BL21(DE3). After induction of the bacteria with isopropyl-beta-D-thiogalactopyranoside (IPTG) pre-nsL-TP was purified from the bacterial lysate by anion exchange chromatography followed by gelfiltration. From 11 of culture, 6-7 mg of pre-nsL-TP was obtained, equal to approximately 7% of the cytoplasmic protein. By use of a fluorescence lipid transfer assay, pre-nsL-TP was found to have lipid transfer activity identical to mature nsL-TP.

引用

页码：179 / 183

页数：5

共 41 条

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