CL- AND NA+ HOMEOSTASIS DURING ANOXIA IN RAT HYPOGLOSSAL NEURONS - INTRACELLULAR AND EXTRACELLULAR INVITRO STUDIES

被引:82
作者
JIANG, C
AGULIAN, S
HADDAD, GG
机构
[1] Department of Pediatrics, Yale University School of Medicine, New Haven, Connecticut
来源
JOURNAL OF PHYSIOLOGY-LONDON | 1992年 / 448卷
关键词
D O I
10.1113/jphysiol.1992.sp019065
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
1. To understand the mechanisms which lead to acute neuronal swelling during anoxia, we studied the ionic movements of Cl- and Na+ during O2 deprivation in the hypoglossal (XII) neurons of rat brain slices using double-barrelled ion-selective microelectrodes. 2. Baseline extracellular Cl- and Na+ activities ([Cl-]o, [Na+]o) were 128.3 +/- 7.4 and 150.0 +/- 3.4 mM respectively (n = 12) in the adult. Similar baseline values were obtained from neonatal brain slices. 3. During a period of anoxia (4 min), [Na+]o decreased by about 40 mM in adult slices while [Na+]o did not show any significant change in the neonate (n = 12). Although anoxia induced a significant decrease of [Cl-]o in both adult and neonate, [Cl-]o dropped 7 times more in the adult than in the neonate (n = 12). 4. Intracellular Cl- activity ([Cl-]i) was studied in twenty-seven adult hypoglossal cells. All showed an increase in [Cl-]i with O2 deprivation. Detailed analysis carried out on ten hypoglossal neurons showed a baseline [Cl-]i of 11.4 +/- 4.5 mM and an increase in [Cl-]i by 20.6 +/- 7.2 mM during O2 limitation. 5. Baseline [Cl-]i in neonatal XII neurons was similar to that of the adult. Anoxia, however, produced an increase in [Cl-]i by only 4.5 +/- 2.4 mM (n = 7). This increase in [Cl-]i was significantly less than that in the adult (P < 0.001). Prolonged anoxia (6-12 min) in the neonate led to a more substantial increase in [Cl-]i, an observation consistent with the decrease in [Cl-]o after prolonged O2 deprivation. 7. We conclude that during anoxia: (1) intracellular [Cl-] increases in the adult and this most likely occurs because of entry of extracellular Cl- into the cytosol and (2) there is a major maturational difference in mechanisms regulating Cl- and Na+ homeostasis between newborn and adult brain tissue. We speculate taht these mechanisms may account, at least partially, for the relative tolerance to anoxia in the newly born.
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收藏
页码:697 / 708
页数:12
相关论文
共 31 条
  • [21] TEMPERATURE-DEPENDENCE OF EXTRACELLULAR IONIC CHANGES EVOKED BY ANOXIA IN HIPPOCAMPAL SLICES
    MORRIS, ME
    LEBLOND, J
    AGOPYAN, N
    KRNJEVIC, K
    [J]. JOURNAL OF NEUROPHYSIOLOGY, 1991, 65 (02) : 157 - 167
  • [22] BETA-ADRENOCEPTOR-MEDIATED DEPOLARIZATION OF THE RESTING MEMBRANE IN GUINEA-PIG PAPILLARY-MUSCLES - CHANGES IN INTRACELLULAR NA+, K+ AND CL- ACTIVITIES
    NAKAYA, H
    HATTORI, Y
    TOHSE, N
    SHIDA, S
    KANNO, M
    [J]. PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1990, 417 (02): : 185 - 193
  • [23] ANION PERMEABILITY IN SPREADING DEPRESSION INVESTIGATED WITH ION-SENSITIVE MICRO-ELECTRODES
    PHILLIPS, JM
    NICHOLSON, C
    [J]. BRAIN RESEARCH, 1979, 173 (03) : 567 - 571
  • [24] RANSOM BR, 1985, J NEUROSCI, V5, P532
  • [25] ROTHMAN SM, 1985, J NEUROSCI, V5, P1483
  • [26] SIESJO BK, 1989, ANN NY ACAD SCI, V568, P234
  • [27] SIESJO BK, 1988, ANN NY ACAD SCI, V522, P638
  • [28] SIGGINS GR, 1986, HDB PHYSL 1, V4, P1
  • [29] A STUDY OF EXTRACELLULAR SPACE IN CENTRAL NERVOUS TISSUE BY FREEZE-SUBSTITUTION
    VANHARREVELD, A
    CROWELL, J
    MALHOTRA, SK
    [J]. JOURNAL OF CELL BIOLOGY, 1965, 25 (1P1) : 117 - +
  • [30] VANHARREVELD A, 1966, BRAIN TISSUE ELECTRO