TRIGONAL CRYSTALS OF PORIN FROM ESCHERICHIA-COLI

被引：36

作者：

PAUPTIT, RA ^{[1
]}

ZHANG, H ^{[1
]}

RUMMEL, G ^{[1
]}

SCHIRMER, T ^{[1
]}

JANSONIUS, JN ^{[1
]}

ROSENBUSCH, JP ^{[1
]}

机构：

[1] UNIV BASEL,BIOCTR,DEPT STRUCT BIOL,CH-4056 BASEL,SWITZERLAND

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1991年 / 218卷 / 03期

关键词：

D O I：

10.1016/0022-2836(91)90696-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Trigonal crystals of the integral membrane protein porin from Escherichia coli have been grown and characterized. They belong to space group P321 with unit cell constants a = b = 118.4, c = 52.7 A ̊, α = β = 90{ring operator}, γ = 120{ring operator}. The crystals grow as well-defined hexagonal prisms to a size of 0.25 mm in all dimensions, and diffract to 2.7 Å. The molecular symmetry coincides with 3-fold crystallographic symmetry, giving two trimers per unit cell (1 monomer/asymmetric unit). This corresponds to VM = 2.9A ̊3/Da. Native X-ray data to 3.0 Å resolution have been collected on a FAST area detector and a search for heavy atom derivatives is underway. © 1991.

引用

页码：505 / 507

页数：3

共 20 条

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