THE STRUCTURE OF THE 5' TERMINAL CAP OF THE RESPIRATORY SYNCYTIAL VIRUS MESSENGER-RNA

被引：40

作者：

BARIK, S

机构：

[1] Department Molecular Biology NC2-130, The Cleveland Clinic Foundation, Cleveland, OH 44195

来源：

JOURNAL OF GENERAL VIROLOGY | 1993年 / 74卷

关键词：

D O I：

10.1099/0022-1317-74-3-485

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The 5' terminal cap structure of the mRNAs of human respiratory syncytial virus synthesized in vitro in the presence of S-adenosyl-L-methionine consists of a 7-methyl guanosine linked to an unmethylated guanosine through a 5'-5' pyrophosphate linkage formed by using the alpha and beta phosphates of GTP. The complete cap structure is m7G (5')ppp(5')Gp ... which is devoid of ribose 2'-O-methylation. Capping, including methylation, is coupled to transcription. These results constitute the first report of a pneumoviral mRNA cap structure.

引用

页码：485 / 490

页数：6

共 20 条

[1] 5' TERMINAL STRUCTURE OF METHYLATED MESSENGER-RNA SYNTHESIZED INVITRO BY VESICULAR STOMATITIS-VIRUS [J].

ABRAHAM, G ;

RHODES, DP ;

BANERJEE, AK .

CELL, 1975, 5 (01) :51-58

[2] NATURE OF RNA PRODUCTS SYNTHESIZED INVITRO BY SUBVIRAL COMPONENTS OF VESICULAR STOMATITIS-VIRUS [J].

ABRAHAM, G ;

BANERJEE, AK .

VIROLOGY, 1976, 71 (01) :230-241

[3] GUANOSINE-5'-DIPHOSPHATE AT 5' TERMINI OF REOVIRUS RNA - EVIDENCE FOR A SEGMENTED GENOME WITHIN VIRION [J].

BANERJEE, AK ;

SHATKIN, AJ .

JOURNAL OF MOLECULAR BIOLOGY, 1971, 61 (03) :643-&

[4] REPLICATION OF RNA VIRUSES .X. REPLICATION OF A NATURAL 6-S RNA BY QBETA RNA POLYMERASE [J].

BANERJEE, AK ;

RENSING, U ;

AUGUST, JT .

JOURNAL OF MOLECULAR BIOLOGY, 1969, 45 (02) :181-&

[5] 5'-TERMINAL CAP STRUCTURE IN EUKARYOTIC MESSENGER RIBONUCLEIC-ACIDS [J].

BANERJEE, AK .

MICROBIOLOGICAL REVIEWS, 1980, 44 (02) :175-205

[6]

BANNERJEE AK, 1992, VIROLOGY, V188, P417

[7] TRANSCRIPTION OF HUMAN RESPIRATORY SYNCYTIAL VIRUS GENOME RNA INVITRO - REQUIREMENT OF CELLULAR FACTOR(S) [J].

BARIK, S .

JOURNAL OF VIROLOGY, 1992, 66 (11) :6813-6818

[8] RIBOSOME BINDING TO REOVIRUS MESSENGER-RNA IN PROTEIN-SYNTHESIS REQUIRES 5' TERMINAL 7-METHYLGUANOSINE [J].

BOTH, GW ;

FURUICHI, Y ;

MUTHUKRISHNAN, S ;

SHATKIN, AJ .

CELL, 1975, 6 (02) :185-195

[9]

COLLINS PL, 1991, PARAMYXOVIRUSES, P103

[10] METHYLATION OF MESSENGER-RNA OF NEWCASTLE-DISEASE VIRUS INVITRO BY A VIRION-ASSOCIATED ENZYME [J].

COLONNO, RJ ;

STONE, HO .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1975, 72 (07) :2611-2615

← 1 2 →