POLIOVIRUS-SPECIFIC MAJOR HISTOCOMPATIBILITY COMPLEX CLASS-I-RESTRICTED CYTOLYTIC T-CELL EPITOPES IN MICE LOCALIZE TO NEUTRALIZING ANTIGENIC REGIONS

A major histocompatibility complex (MHC) class I-restricted cytotoxic T-lymphocyte (CTL) response is induced in BALB/c mice upon immunization with poliovirus serotype 1 (Mahoney strain). A similar class I-restricted response is also induced upon immunization with purified VP1 capsid proteins. Thus, poliovirus-specific MHC class I CTL responses can be induced independently of viral infection in murine hosts. In experiments using recombinant vaccinia virus vectors expressing different segments of the poliovirus capsid proteins and synthetic peptides, two regions of the VPI capsid protein appear to contain epitopes recognized by this bulk CTL population. These epitope regions contain a K(d)-restricted peptide-binding motif. Interestingly, each of these CTL epitopes is located near previously defined neutralizing antigenic sites.