DEVELOPMENTAL EXPRESSION OF THE GROWTH-HORMONE GENE IN THE GILTHEAD SEA BREAM SPARUS-AURATA

被引：17

作者：

FUNKENSTEIN, B ^{[1
]}

TANDLER, A ^{[1
]}

CAVARI, B ^{[1
]}

机构：

[1] ISRAEL OCEANOG & LIMNOL RES LTD,NATL CTR MARICULTURE,ELAT,ISRAEL

来源：

MOLECULAR AND CELLULAR ENDOCRINOLOGY | 1992年 / 87卷 / 1-3期

关键词：

FISH; LARVA; GROWTH HORMONE; RNA BLOT HYBRIDIZATION; BETA-ACTIN;

D O I：

10.1016/0303-7207(92)90249-6

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Expression of growth hormone (GH) gene during early stages of larval development of the teleost Sparus aurata was determined by Northern blot analysis. Poly(A+) RNA was prepared from a pool of larvae collected on different days after hatching. When hybridized to Sparus aurata GH cDNA, GH specific mRNA was first seen on day 6 post-hatching. In contrast, the levels of beta-actin mRNA, which was used to normalize for RNA amounts, were already high on the day of hatching. Our results suggest that expression of the GH gene is very low immediately after hatching, and increases dramatically within 6 days.

引用

页码：R7 / R9

页数：3

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