PRODUCTION OF BETA-GLUCOSIDASE (CELLOBIASE) BY CURVULARIA SP

被引：10

作者：

BANERJEE, UC

机构：

[1] Institute of Microbial Technology, Chandigarh, 160031, Kothi No. 1389

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1990年 / 10卷 / 05期

关键词：

D O I：

10.1111/j.1472-765X.1990.tb01332.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A Curvularia sp. isolated from soil was found to produce extracellular β‐glucosidase activity when grown in yeast extract, peptone, carboxymethylcellulose (YPC) medium. An initial medium pH of 6·5 and cultivation temperature of 30°C were found to be most suitable for high enzyme productivity. The pH and temperature optima for the enzyme were 4·0 and 70°C, respectively. Under these conditions, the enzyme exhibited a Km (0‐nitrophenyl‐β‐d‐glucoside) value of 0.20 mmol/l. Several divalent metal ions inhibited enzyme activity at high concentration. EDTA. also inhibited β‐glucosidase activity. Copyright © 1990, Wiley Blackwell. All rights reserved

引用

页码：197 / 199

页数：3

共 9 条

[1]

AUNSTRUP K, 1978, ANN REPORTS FERMENTA, V2, P124

[2]

EVANS CS, 1985, APPL MICROBIOL BIOT, V22, P128

[3] YEAST BETA-GLUCOSIDASE - COMPARISON OF THE PHYSICAL-CHEMICAL PROPERTIES OF PURIFIED CONSTITUTIVE AND INDUCIBLE ENZYME [J].