PEANUT AGGLUTININ .4. TOOL FOR STUDYING HUMAN MONONUCLEAR CELL-DIFFERENTIATION

Peanut agglutinin (PNA) has previously been shown to be a ‘marker’ for early T subpopulations in mice. We have investigaied whether it could also be used in the study of human mononuclear cells. 50–60% of human thymocytes have binding sites for PNA. When separated on a discontinuous Ficoll gradient, the PNA‐positive thymocytes are found preferentially in the layers corresponding to the immunoincompetent cells. In the peripheral blood only 5% of the mononuclear cells are PNA‐positive and we have shown that these cells are monocytes. In tonsils 13% of PNA+ cells are found and they are mostly lymphocytes. Thus, PNA is a marker for some T cell subsets present in the thymus and tonsils. Copyright © 1979, Wiley Blackwell. All rights reserved