STRUCTURAL-ANALYSIS OF OLIGOSACCHARIDE BISPHOSPHATES ISOLATED FROM THE LIPOPOLYSACCHARIDE OF A RECOMBINANT STRAIN OF ESCHERICHIA-COLI F515 (RE CHEMOTYPE) EXPRESSING THE GENUS-SPECIFIC EPITOPE OF CHLAMYDIA LIPOPOLYSACCHARIDE

被引：89

作者：

HOLST, O ^{[1
]}

BROER, W ^{[1
]}

THOMASOATES, JE ^{[1
]}

MAMAT, U ^{[1
]}

BRADE, H ^{[1
]}

机构：

[1] UNIV UTRECHT,BIJVOET CTR BIOMOLEC RES,DEPT MASS SPECTROMETRY,UTRECHT,NETHERLANDS

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1993年 / 214卷 / 03期

关键词：

D O I：

10.1111/j.1432-1033.1993.tb17971.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The lipopolysaccharide of the recombinant strain Escherichia coli F515-207, expressing the genus-specific epitope of Chlamydia lipopolysaccharide, was sequentially de-O- and de-N-acylated by mild hydrazinolysis and treatment with 4 M KOH, respectively, yielding two oligosaccharide bisphosphates which were isolated by high-performance anion-exchange chromatography and gel-permeation chromatography. Their structures were determined by chemical analysis, NMR spectroscopy, and mass spectrometry as alpha-Kdo-(2-4)-alpha-Kdo-(2-6)-beta-D-GlcN-(1-6)-alpha-D-GlcN 1,4'-P, (tetrasaccharide bisphosphate) and alpha-Kdo-(2-8)-alpha-Kdo-(2-4)-alpha-Kdo-(2-6)-beta-D-GlcN-(1-6 )-alpha-D-GlcN 1,4'-P2 (pentasaccharide bisphosphate).

引用

页码：703 / 710

页数：8

共 46 条

[1] CHARGE-REMOTE FRAGMENTATIONS - ANALYTICAL APPLICATIONS AND FUNDAMENTAL-STUDIES
ADAMS, J
[J]. MASS SPECTROMETRY REVIEWS, 1990, 9 (02) : 141 - 186
[2] BELUNIS CJ, 1992, J BIOL CHEM, V267, P18702
[3] C-13 NUCLEAR MAGNETIC-RESONANCE SPECTROSCOPY OF MONOSACCHARIDES
BOCK, K
PEDERSEN, C
[J]. ADVANCES IN CARBOHYDRATE CHEMISTRY AND BIOCHEMISTRY, 1983, 41 : 27 - 66
[4] STUDY OF C-13H COUPLING-CONSTANTS IN HEXOPYRANOSES
BOCK, K
PEDERSEN, C
[J]. JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 2, 1974, (03): : 293 - 299
[5] A NUCLEAR-MAGNETIC-RESONANCE SPECTROSCOPIC INVESTIGATION OF KDO-CONTAINING OLIGOSACCHARIDES RELATED TO THE GENUS-SPECIFIC EPITOPE OF CHLAMYDIA LIPOPOLYSACCHARIDES
BOCK, K
THOMSEN, JU
KOSMA, P
CHRISTIAN, R
HOLST, O
BRADE, H
[J]. CARBOHYDRATE RESEARCH, 1992, 229 (02) : 213 - 224
[6] CHEMICAL AND SEROLOGICAL INVESTIGATIONS ON THE GENUS-SPECIFIC LIPOPOLYSACCHARIDE EPITOPE OF CHLAMYDIA
BRADE, H
BRADE, L
NANO, FE
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (08) : 2508 - 2512
[7] SPECTROSCOPIC ANALYSIS OF A 3-DEOXY-D-MANNO-2-OCTULOSONIC ACID (KDO)-DISACCHARIDE FROM THE LIPOPOLYSACCHARIDE OF A SALMONELLA-GODESBERG RE MUTANT
BRADE, H
ZAHRINGER, U
RIETSCHEL, ET
CHRISTIAN, R
SCHULZ, G
UNGER, FM
[J]. CARBOHYDRATE RESEARCH, 1984, 134 (01) : 157 - 166
[8] ANTIGENIC AND IMMUNOGENIC PROPERTIES OF RECOMBINANTS FROM SALMONELLA-TYPHIMURIUM AND SALMONELLA-MINNESOTA ROUGH MUTANTS EXPRESSING IN THEIR LIPOPOLYSACCHARIDE A GENUS-SPECIFIC CHLAMYDIAL EPITOPE
BRADE, L
NANO, FE
SCHLECHT, S
SCHRAMEK, S
BRADE, H
[J]. INFECTION AND IMMUNITY, 1987, 55 (02) : 482 - 486
[9] ANTIGENIC PROPERTIES OF CHLAMYDIA-TRACHOMATIS LIPOPOLYSACCHARIDE
BRADE, L
NURMINEN, M
MAKELA, PH
BRADE, H
[J]. INFECTION AND IMMUNITY, 1985, 48 (02) : 569 - 572
[10] CHEMICAL, BIOLOGICAL, AND IMMUNOCHEMICAL PROPERTIES OF THE CHLAMYDIA-PSITTACI LIPOPOLYSACCHARIDE
BRADE, L
SCHRAMEK, S
SCHADE, U
BRADE, H
[J]. INFECTION AND IMMUNITY, 1986, 54 (02) : 568 - 574

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