STRUCTURAL-ANALYSIS OF OLIGOSACCHARIDE BISPHOSPHATES ISOLATED FROM THE LIPOPOLYSACCHARIDE OF A RECOMBINANT STRAIN OF ESCHERICHIA-COLI F515 (RE CHEMOTYPE) EXPRESSING THE GENUS-SPECIFIC EPITOPE OF CHLAMYDIA LIPOPOLYSACCHARIDE

被引：89

作者：

HOLST, O ^{[1
]}

BROER, W ^{[1
]}

THOMASOATES, JE ^{[1
]}

MAMAT, U ^{[1
]}

BRADE, H ^{[1
]}

机构：

[1] UNIV UTRECHT,BIJVOET CTR BIOMOLEC RES,DEPT MASS SPECTROMETRY,UTRECHT,NETHERLANDS

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1993年 / 214卷 / 03期

关键词：

D O I：

10.1111/j.1432-1033.1993.tb17971.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The lipopolysaccharide of the recombinant strain Escherichia coli F515-207, expressing the genus-specific epitope of Chlamydia lipopolysaccharide, was sequentially de-O- and de-N-acylated by mild hydrazinolysis and treatment with 4 M KOH, respectively, yielding two oligosaccharide bisphosphates which were isolated by high-performance anion-exchange chromatography and gel-permeation chromatography. Their structures were determined by chemical analysis, NMR spectroscopy, and mass spectrometry as alpha-Kdo-(2-4)-alpha-Kdo-(2-6)-beta-D-GlcN-(1-6)-alpha-D-GlcN 1,4'-P, (tetrasaccharide bisphosphate) and alpha-Kdo-(2-8)-alpha-Kdo-(2-4)-alpha-Kdo-(2-6)-beta-D-GlcN-(1-6 )-alpha-D-GlcN 1,4'-P2 (pentasaccharide bisphosphate).

引用

页码：703 / 710

页数：8

共 46 条

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