RE-FACE SPECIFICITY AT C14A OF METHYLENETETRAHYDROMETHANOPTERIN AND SI-FACE SPECIFICITY AT C5 OF COENZYME F-420 FOR COENZYME F-420-DEPENDENT METHYLENETETRAHYDROMETHANOPTERIN DEHYDROGENASE FROM METHANOGENIC ARCHAEA

被引：24

作者：

KLEIN, AR

THAUER, RK

机构：

[1] MAX PLANCK INST TERR MIKROBIOL, D-35043 MARBURG, GERMANY

[2] UNIV MARBURG, FACHBEREICH BIOL, MIKROBIOL LAB, MARBURG, GERMANY

来源：

EUROPEAN JOURNAL OF BIOCHEMISTRY | 1995年 / 227卷 / 1-2期

关键词：

COENZYME F-420; STEREOSPECIFICITY; METHYLENETETRAHYDROMETHANOPTERIN DEHYDROGENASE; HYDROGENASE;

D O I：

10.1111/j.1432-1033.1995.tb20373.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Coenzyme F-420-dependent methylenetetrahydromethanopterin dehydrogenase from methanogenic Archaea catalyzes the reversible transfer of a hydride ion from C14a of N-5,N-10-methylenetetrahydromethanopterin to C5 of coenzyme F-420. In this study, we report that this hydride transfer proceeds stereospecifically from the Re face at C14a to the Si face at C5. The results were obtained by using chirally H-3-labelled N-5,N-10-methylenetetrahydromethanopterin generated via Re-face-specific H-2-forming N-5,N-10-methylenetetrahydromethanopterin dehydrogenase and by analyzing reduced coenzyme F-420 via Si-face-specific F-420-reducing hydrogenase.

引用

页码：169 / 174

页数：6

共 46 条

[31] ACTIVITIES OF FORMYLMETHANOFURAN DEHYDROGENASE, METHYLENETETRAHYDROMETHANOPTERIN DEHYDROGENASE, METHYLENETETRAHYDROMETHANOPTERIN REDUCTASE, AND HETERODISULFIDE REDUCTASE IN METHANOGENIC BACTERIA
SCHWORER, B
THAUER, RK
[J]. ARCHIVES OF MICROBIOLOGY, 1991, 155 (05) : 459 - 465
[32] H-2-FORMING N5,N10-METHYLENETETRAHYDROMETHANOPTERIN DEHYDROGENASE FROM METHANOBACTERIUM-THERMOAUTOTROPHICUM - STUDIES OF THE CATALYTIC MECHANISM OF H-2 FORMATION USING HYDROGEN ISOTOPES
SCHWORER, B
FERNANDEZ, VM
ZIRNGIBL, C
THAUER, RK
[J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1993, 212 (01): : 255 - 261
[33] SYNTHESIS OF (6R,11S) AND (6R,11R)-5,10-METHYLENE[11-H-1,H-2]TETRAHYDROFOLATE - STEREOCHEMICAL PATHS OF SERINE HYDROXYMETHYLTRANSFERASE, 5,10-METHYLENETETRAHYDROFOLATE DEHYDROGENASE, AND THYMIDYLATE SYNTHETASE CATALYSIS
SLIEKER, LJ
BENKOVIC, SJ
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1984, 106 (06) : 1833 - 1838
[34] STEREOCHEMISTRY AND MECHANISM OF HYDROGEN TRANSFER BETWEEN NADPH AND METHYLENETETRAHYDROFOLATE IN THE REACTION CATALYZED BY METHYLENETETRAHYDROFOLATE REDUCTASE FROM PIG-LIVER
SUMNER, JS
MATTHEWS, RG
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1992, 114 (18) : 6949 - 6956
[35] PURIFICATION AND PROPERTIES OF 5,10-METHYLENETETRAHYDROMETHANOPTERIN DEHYDROGENASE AND 5,10-METHYLENETETRAHYDROMETHANOPTERIN REDUCTASE, 2 COENZYME-F420-DEPENDENT ENZYMES, FROM METHANOSARCINA-BARKERI
TEBROMMELSTROET, BWJ
GEERTS, WJ
KELTJENS, JT
VANDERDRIFT, C
VOGELS, GD
[J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1991, 1079 (03) : 293 - 302
[36] ELUCIDATION OF STEREOSPECIFICITY OF A SELENIUM-CONTAINING HYDROGENASE FROM METHANOCOCCUS-VANNIELII - SYNTHESIS OF (R) AND (S)-[4-2H1]-3,4-DIHYDRO-7-HYDROXY-1-HYDROXYETHYLQUINOLINONE
TESHIMA, T
NAKAJI, A
SHIBA, T
TSAI, L
YAMAZAKI, S
[J]. TETRAHEDRON LETTERS, 1985, 26 (03) : 351 - 354
[37] THAUER RK, 1995, IN PRESS BIOTECHNOLO
[38] 5,10-METHENYL-5,6,7,8-TETRAHYDROMETHANOPTERIN, A ONE-CARBON CARRIER IN THE PROCESS OF METHANOGENESIS
VANBEELEN, P
VANNECK, JW
DECOCK, RM
VOGELS, GD
GUIJT, W
HAASNOOT, CAG
[J]. BIOCHEMISTRY, 1984, 23 (19) : 4448 - 4454
[39] VONBUNAU R, 1991, EUR J BIOCHEM, V202, P1205
[40] VORHOLT J, 1994, IN PRESS ARCH MICROB

← 1 2 3 4 5 →