MOLECULAR-CLONING AND FUNCTIONAL EXPRESSION OF THE 2ND MOUSE NM23/NDP KINASE GENE, NM23-M2

被引：86

作者：

URANO, T

TAKAMIYA, K

FURUKAWA, K

SHIKU, H

机构：

[1] NAGASAKI UNIV,SCH MED,DEPT ONCOL,12-4 SAKAMOTO MACHI,NAGASAKI 852,JAPAN

[2] NAGASAKI UNIV,SCH MED,DEPT PATHOL 1,NAGASAKI 852,JAPAN

来源：

FEBS LETTERS | 1992年 / 309卷 / 03期

关键词：

NM23/NUCLEOSIDE DIPHOSPHATE KINASE; METASTATIC SUPPRESSOR GENE; NM23-M2; GENE; MONOCLONAL ANTIBODY;

D O I：

10.1016/0014-5793(92)80807-S

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new murine cDNA of nm23/NDP kinase was isolated. A RT-PCR product was obtained from the normal mouse liver mRNA with primers designed for the human nm23-H2 gene. The product was used as a probe to screen a cDNA library from the murine melanoma cell line, B16, and two clones containing the entire open reading frame were obtained. It was predicted that the DNA sequence encoded 152 amino acids which was 98% identical to the nm23-H2 protein. The entire nm23-M1 and -M2 gene-coding regions were translated as fusion proteins with a glutathione S-transferase. These fusion proteins displayed NDP kinase activities.

引用

页码：358 / 362

页数：5

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