AN ESSENTIAL ROLE FOR THE ESCHERICHIA-COLI DNAK PROTEIN IN STARVATION-INDUCED THERMOTOLERANCE, H2O2 RESISTANCE, AND REDUCTIVE DIVISION

被引：54

作者：

ROCKABRAND, D ^{[1
]}

ARTHUR, T ^{[1
]}

KORINEK, G ^{[1
]}

LIVERS, K ^{[1
]}

BLUM, P ^{[1
]}

机构：

[1] UNIV NEBRASKA,SCH BIOL SCI,LINCOLN,NE 68588

来源：

JOURNAL OF BACTERIOLOGY | 1995年 / 177卷 / 13期

关键词：

D O I：

10.1128/jb.177.13.3695-3703.1995

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

During a 3-day period, glucose starvation of wild-type Escherichia coli produced thermotolerant, H2O2-resistant, small cells with a round morphology. These cells contained elevated levels of the DnaK protein, adjusted either for total protein or on a per-cell basis. Immunoprecipitation of [S-35]methionine-labeled protein produced by such starving cells demonstrated that DnaK underwent continuous synthesis but at decreasing rates throughout this time. Glucose resupplementation of starving cells resulted in rapid loss of thermotolerance, H2O2 resistance, and the elevated DnaK levels. A dnaK deletion mutant, but not ad otherwise isogenic wild-type strain, failed to develop starvation-induced thermotolerance or H2O2 resistance. The filamentous phenotype associated with DnaK deficiency was suppressed by cultivation in a defined glucose medium. When starved for glucose, the nonfilamentous and rod-shaped dnaK mutant strain failed to convert into the small spherical form typical of starving wild-type cells. The dnaK mutant retained the ability to develop adaptive H2O2 resistance during growth but not adaptive resistance to heat. Complementation of DnaK deficiency by using P-tac-regulated dnaK(+) and dnaK(+)J(+) expression plasmids confirmed a specific role for the DnaK molecular chaperone in these starvation-induced phenotypes.

引用

页码：3695 / 3703

页数：9

共 57 条

[1]

Aldea M., Garrido T., Hernandez-Chico C., Vincente M., Kushner S.R., Induction of a growth-phase-dependent promoter triggers transcription of bolA, an Escherichia coli morphogene, EMBO J., 8, pp. 3923-3931, (1989)

[2]

Almiron M., Link A.J., Furlong D., Kolter R., A novel DNAbinding protein with regulatory and protective role in starved Escherichia coli, Genes Dev., 6, pp. 2646-2654, (1992)

[3]

Blum P., Ory J., Bauernfeind J., Krska J., Physiological consequences of DnaK and DnaJ overproduction in Escherichia coli, J. Bacteriol., 174, pp. 7436-7444, (1992)

[4]

Blum P.H., Jovanovich S.B., McCann M.P., Schultz J.E., Lesley S.A., Burgess R.R., Matin A., Cloning and in vivo and in vitro regulation of cyclic AMP-dependent carbon starvation genes from Escherichia coli, J. Bacteriol., 172, pp. 3813-3820, (1990)

[5]

Bukau B., Walker G.C., ΔdnaK52 mutants of Escherichia coli have defects in chromosome segregation and plasmid maintenance at normal growth temperatures, J. Bacteriol., 171, pp. 6030-6038, (1989)

[6]

Bukau B., Walker G.C., Cellular defects caused by deletion of the Escherichia coli dnaK gene indicate roles for heat shock protein in normal metabolism, J. Bacteriol., 171, pp. 2337-2346, (1989)

[7]

Bukau B., Walker G.C., Mutations altering heat shock specific subunit of RNA polymerase suppress major cellular defects of E. coli mutants lacking the DnaK chaperone, EMBO J., 9, pp. 4027-4036, (1990)

[8]

Burkholder W.F., Panagiotidis C.A., Silverstein S.J., Cegielska A., Gottesman M.E., Gaitanaris G.A., Isolation and characterization of an Escherichia coli DnaK mutant with impaired ATPase activity, J. Mol. Biol., 242, pp. 364-377, (1994)

[9]

Dean D.O., James R., Identification of a gene, closely linked to dnaK, which is required for high-temperature growth of Escherichia coli, J. Gen. Microbiol., 137, pp. 1271-1277, (1991)

[10]

Delaney J.M., Requirement of the Escherichia coli dnaK gene for thermotolerance and protection against H<sub>2</sub>O<sub>2</sub> , J. Gen. Microbiol., 136, pp. 2113-2118, (1990)

← 1 2 3 4 5 6 →