PULSE NUCLEAR MAGNETIC-RESONANCE MEASUREMENTS OF WATER EXCHANGE ACROSS THE ERYTHROCYTE-MEMBRANE EMPLOYING A LOW MN CONCENTRATION

被引：84

作者：

PIRKLE, JL ^{[1
]}

ASHLEY, DL ^{[1
]}

GOLDSTEIN, JH ^{[1
]}

机构：

[1] EMORY UNIV,SCH MED,ATLANTA,GA 30322

来源：

BIOPHYSICAL JOURNAL | 1979年 / 25卷 / 03期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/S0006-3495(79)85311-4

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A simple, precise, and rapid pulse nuclear magnetic resonance technique for measuring the rate of water exchange across the erythrocyte membrane is presented. The technique is based upon the nonlinear fit of Carr-Purcell-Meiboom-Gill (CPMG) transverse relaxation time data of blood doped with 1.7 mM MnCl2 to the general two-compartment exchange condition. Previous approaches using CPMG data required high MnCl2 concentrations (25–53 nM), shown in this work to induce systematic errors ranging from 35 to 45%. At 23 degrees C the average residence time of a water molecule inside the erythrocyte (tau a) is 21.0 +/- 0.6 ms (SE). The Arrhenius plot for water exchange is linear over the range of 3 degrees - 37 degrees C and th Arrhenius activation energy is 4.79 +/- 0.03 kcal (SE). This value does not differ significantly from the energy required for bulk water flow. Results are compared with previous determinations, and sources of systematic error in tau a and the activation energy are evaluated. © 1979, The Biophysical Society. All rights reserved.

引用

页码：389 / 406

页数：18

共 51 条

[11] WATER DIFFUSION PERMEABILITY OF ERYTHROCYTES USING AN NMR TECHNIQUE [J].