DNA-HYBRIDIZATION ELECTRON-MICROSCOPY - LOCALIZATION OF 5 REGIONS OF 16-S RIBOSOMAL-RNA ON THE SURFACE OF 30-S RIBOSOMAL-SUBUNITS

被引：46

作者：

OAKES, MI ^{[1
]}

LAKE, JA ^{[1
]}

机构：

[1] UNIV CALIF LOS ANGELES,DEPT BIOL,LOS ANGELES,CA 90024

来源：

JOURNAL OF MOLECULAR BIOLOGY | 1990年 / 211卷 / 04期

关键词：

D O I：

10.1016/0022-2836(90)90082-W

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

DNA-hybridization electron microscopy has been used to locate five regions of 16 S rRNA on the surface of 30 S ribosomal subunits. Biotinylated DNA probes that are complementary to selected regions of 16 S rRNA were hybridized to activated 30 S ribosomal subunits. These hybridized probes were reacted with avidin and localized by electron microscopy. The specificity of DNA binding was monitored with RNase H, which recognizes RNA-DNA hybrids and cleaves the RNA. Three of the five sequences examined were mapped on the platform. These sequences are 686-703, 714-733 and 787-803. Region 1492-1505 is mapped in the cleft and region 518-533 is at the neck on the side opposite the platform, respectively. © 1990.

引用

页码：897 / 906

页数：10

共 43 条

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