INTEGRIN RECEPTORS AND PLATELET-ADHESION TO SYNTHETIC SURFACES

被引:44
作者
GOODMAN, SL
COOPER, SL
ALBRECHT, RM
机构
[1] UNIV WISCONSIN, DEPT BIOMED SCI, MADISON, WI 53706 USA
[2] UNIV DELAWARE, COLL ENGN, NEWARK, DE 19716 USA
来源
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH | 1993年 / 27卷 / 05期
关键词
D O I
10.1002/jbm.820270516
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
The activation-independent and -dependent integrin receptors-glycoproteins GPIc-IIa(alpha5-beta1) and GPIIb-IIIa (alpha(IIb)-beta3)-are involved in platelet adhesion and thrombus growth on damaged subendothelium through interactions with fibrinogen, fibronectin, von Willebrand factor, and other adhesive proteins. Because these receptors are used in normal in vivo hemostatic adhesion, they may also have a role for adhesion onto synthetic surfaces in the vasculature. Platelet adhesion in vitro was examined onto Formvar, glass, and four polyurethaneureas with various soft segment chemistries and surface properties. Platelets were pretreated with RGD peptides before and after adhesion. RGD peptide pretreatment inhibited spreading and close contact formation compared to treatment with saline or control RGE peptides, with no observable effect on the number of adherent platelets per area. High-voltage electron microscopy showed abnormally sparse and short microfilament structures with RGD peptide treatment, suggesting an indirect inhibition of actin filament formation. Video-enhanced light microscopy showed a cessation of spreading and a partial reversal of close contacts following RGD peptide application to adherent platelets. Because minimal amounts of plasma proteins are present in column-washed platelet suspensions, and as platelet secretion appeared to be minimal in these experiments, these observations suggest that RGD binding integrin receptors may function in platelet spreading even in the absence of exogenous ligand. As RGD peptides did not affect the numbers of adherent platelets, while producing substantial decreases in the extent of spreading, we suggest that platelet integrins, possibly GPIIb-IIIa, are involved in spreading on synthetic Surfaces but not for initial adhesion.
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页码:683 / 695
页数:13
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