2 GENES ENCODING MINOR LEGUMIN POLYPEPTIDES IN PEA (PISUM-SATIVUM-L) - CHARACTERIZATION AND COMPLETE SEQUENCE OF THE LEGJ GENE

被引：36

作者：

GATEHOUSE, JA ^{[1
]}

BOWN, D ^{[1
]}

GILROY, J ^{[1
]}

LEVASSEUR, M ^{[1
]}

CASTLETON, J ^{[1
]}

ELLIS, THN ^{[1
]}

机构：

[1] JOHN INNES INST, NORWICH NR4 7UH, NORFOLK, ENGLAND

来源：

BIOCHEMICAL JOURNAL | 1988年 / 250卷 / 01期

关键词：

D O I：

10.1042/bj2500015

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A genomic clone from pea (Pisum sativum L.) contains all of one gene encoding a ''minor'' (B-type) legumin polypeptide, and most of a second very similar gene. The two genes, designated LegJ and LegK, are arranged in tandem, separated by approx. 6 kb. A complete sequence of gene LegJ and its flanking sequences is given with as much of the sequence of gene LegK as is present on the genomic clone. Hybridization of 3'' flanking sequence probes to seed mRNA, and sequence comparisons with cDNA species, suggested that gene LegJ and probably gene LegK, was expressed. The partial amino acid sequences of ''minor'' legumin .alpha.- and .beta.-polypeptides were used to confirm the identity of these genes. The transcription start in gene LegJ was mapped. The 5'' flanking sequence of gene LegJ contains a sequence conserved in legumin genes from pea and other species, which is likely to have functional significance in control of gene expression. Sequence comparisons with legumin genes and cDNA species from Vicia faba and soya bean show that separation of legumin genes into A- and B-type subfamilies occurred before separation of the Viciae and Glycinae tribes.

引用

页码：15 / 24

页数：10

共 43 条

[1] THE LEGUMIN GENE FAMILY - STRUCTURE OF A B-TYPE GENE OF VICIA-FABA AND A POSSIBLE LEGUMIN GENE SPECIFIC REGULATORY ELEMENT [J].