ASSOCIATION OF NEUROTENSIN RECEPTORS WITH VIP-CONTAINING NEURONS AND SEROTONIN-CONTAINING AXONS IN THE SUPRACHIASMATIC NUCLEUS OF THE RAT

被引:24
作者
FRANCOISBELLAN, AM
BOSLER, O
TONON, MC
WEI, LT
BEAUDET, A
机构
[1] MCGILL UNIV,MONTREAL NEUROL INST,NEUROANAT LAB,ROOM 896,3801 UNIV ST,MONTREAL H3A 2B4,QUEBEC,CANADA
[2] CNRS,NEUROBIOL LAB,F-13402 MARSEILLE 9,FRANCE
[3] UNIV ROUEN HAUTE NORMANDIE,CNRS,MOLEC ENDOCRINOL GRP,F-76134 MT ST AIGNAN,FRANCE
关键词
NEUROTENSIN; VIP; RECEPTORS; IMMUNOHISTOCHEMISTRY; AUTORADIOGRAPHY; SUPRACHIASMATIC NUCLEUS;
D O I
10.1002/syn.890100403
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The aim of the present study was to identify cellular elements bearing high-affinity neurotensin (NT) binding sites in the suprachiasmatic nucleus (SCN) of the rat hypothalamus. Because the distribution of these binding sites had previously been reported to conform to that of both vasoactive intestinal peptide (VIP)-containing nerve cell bodies and serotonin (5-HT)-containing axons, the following experimental approaches were used: (1) the overlap between autoradiographically labeled NT binding sites and immunocytochemically labeled VIP neurons was examined in adjacent 5-mu-m-thick sections taken across the entire rostrocaudal extent of the SCN; and (2) the density of NT binding sites was examined by quantitative autoradiography following cytotoxic lesioning of 5-HT afferents. Double-labeling studies demonstrated precise overlap between I-125-NT binding and VIP immunostaining throughout the SCN. Moreover, at high magnification intensely VIP-immunoreactive neurons were found in direct register with I-125-NT-labeled cells visualized in adjacent sections. Densitometric autoradiographic studies demonstrated a significant reduction in specific I-125-NT binding within the SCN following intracerebroventricular injection of the neurotoxin, 5,7-dihydroxytryptamine. Taken together, these results indicate that within the SCN, NT receptors are present both presynaptically on serotonin axons and postsynaptically on the perikarya and dendrites of VIP-containing neurons.
引用
收藏
页码:282 / 290
页数:9
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