CA2+ COOPERATIVITY IN NEUROSECRETION MEASURED USING PHOTOLABILE CA2+ CHELATORS

1. The photolabile Ca2+ chelator DM-nitrophen was injected into crayfish motor neuron terminals and photolyzed with light flashes of different intensity to determine the cooperativity of Ca2+ action in releasing neurotransmitter. 2. Each flash elicited a phasic postsynaptic response resembling an excitatory junctional potential, apparently due to a presynaptic ''spike'' in intracellular calcium concentration ([Ca2+](i)). 3. When postsynaptic currents were measured under voltage clamp, a Ca2+ cooperativity of similar to 3-4 was inferred from a supralinear dependence of responses on changes in peak [Ca2+](i) caused by flashes differing in intensity by 32-46%. 4. A similar Ca2+ cooperativity was inferred from postsynaptic potentials in response to flashes of varying intensity. 5. The time course of transmitter release indicated by flash responses had slightly slower rising and falling phases than excitatory postsynaptic potentials. There was also a slow tail of transmitter release lasting for similar to 200 ms after a flash. 6. This time course was explained quantitatively by simulations of DM-nitrophen photolysis and binding reactions and a model of Ca2+ activation of transmitter release.