DISTRIBUTION OF DECANE WITHIN THE UNIT-CELL OF THE INVERTED HEXAGONAL (HII) PHASE OF LIPID WATER DECANE SYSTEMS DETERMINED BY NEUTRON-DIFFRACTION

The addition of a free alkane such as decane to lipid-water systems is known to promote the formation of a low-temperature inverted hexagonal (H(II)) phase [Kirk, G. L., & Gruner, S. M. (1985) J. Phys. (Paris) 46, 761]. Kirk et al. [Kirk, G. L., Gruner, S. M., & Stein, D. E. (I 984) Biochemistry 23, 1093] have discussed the hydrocarbon packing anisotropy in the H(II) unit cell and have suggested that free alkane will distribute in a way that reduces this packing anisotropy by allowing the lipid chain environment to become more uniform. By combining neutron and X-ray diffraction data to do a Fourier reconstruction of the H(II) phase of dioleoylphosphatidylethanolamine (DOPE) + water + deuterated decane, it was found that the decane preferentially partitions into the interstitial regions of the H(II) unit cell where it should be the most effective in alleviating the hydrocarbon chain packing stress, supporting the suggestion of Kirk et al. Using the distribution of decane within the unit cell, we have calculated the lipid length distribution for the situations with and without added alkane. With a suitable molecular model, this lipid length distribution may eventually be used to calculate the free energy change upon the addition of alkane. Such a measurement is important for a more realistic understanding of the interactions which lead to the formation of the H(II) phase.