THE PROTEIN-PHOSPHATASE INHIBITOR OKADAIC ACID MIMICS MSH-INDUCED AND MELATONIN-REVERSIBLE MELANOSOME DISPERSION IN XENOPUS-LAEVIS MELANOPHORES

被引:26
作者
COZZI, B [1 ]
ROLLAG, MD [1 ]
机构
[1] UNIFORMED SERV UNIV HLTH SCI,DEPT ANAT,4301 JONES BRIDGE RD,BETHESDA,MD 20814
来源
PIGMENT CELL RESEARCH | 1992年 / 5卷 / 03期
关键词
MELANOPHORE; MELATONIN; OKADAIC ACID; MELANOCYTE STIMULATING HORMONE; CALMODULIN; TFP; W7;
D O I
10.1111/j.1600-0749.1992.tb00011.x
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The present study describes the ability of 315 nM okadaic acid to induce melanosome dispersion in cultured Xenopus laevis melanophores. This effect of okadaic acid is similar to that of a-melanocyte stimulating hormone (MSH) and can be reversed by melatonin treatment; it indicates that a member of the protein-phosphatase 1 or 2A families must be active for maintenance of the aggregated state. Higher concentrations of okadaic acid (1-mu-M) attenuate the response of Xenopus melanophores to melatonin leading to the hypothesis that melatonin action is mediated by the calcium/calmodulin activated phosphatase 2B. This hypothesis seems unlikely, however, since the calcium/calmodulin inhibitors TFP and W7 do not prevent melatonin-induced pigment aggregation, but instead induce aggregation on their own.
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页码:148 / 154
页数:7
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