MICRO-ELECTROSPRAY - ZEPTOMOLE-ATTOMOLE PER MICROLITER SENSITIVITY FOR PEPTIDES

被引：109

作者：

ANDREN, PE

EMMETT, MR

CAPRIOLI, RM

机构：

[1] UNIV TEXAS,SCH MED,DEPT BIOCHEM & MOLEC BIOL,6431 FANNIN ST,HOUSTON,TX 77225

[2] UNIV TEXAS,SCH MED,CTR ANALYT CHEM,HOUSTON,TX 77225

来源：

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY | 1994年 / 5卷 / 09期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/1044-0305(94)87010-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The micro-electrospray ionization source has been optimized for the specific analysis of neuropeptides such as neurotensin and methionine enkephalin. The source has the option of integrating nanoliter flow-rate desalting and preconcentration techniques into the micro-electrospray spray needle, eliminating post-column dead volumes. For neurotensin, the most sensitive neuropeptide analyzed thus far in this work, the injection of 10 muL of a solution containing 320 zeptomoles /muL gave an [M + 3H]+3 ion at m/z 558.4 with S/N of > 8:1. The MS/MS analysis of this peptide for the fragment ion at m/z 578.9 gave a S/N > 20:1 for a solution containing 32 attomoles /muL.

引用

页码：867 / 869

页数：3