PITUITARY-TESTICULAR AXIS IN RATS LACKING TESTICULAR MACROPHAGES

Testicular macrophages were depleted selectively in adult rats by intratesticular injections of liposome-entrapped dichloromethylene diphosphonate (Cl2MDP-lp), whereas control animals received intratesticular injections of phosphate-buffered saline-containing liposomes or 0.9% NaCl. The absence of macrophages in Cl2MDP-lp-injected rats was confirmed histologically. Rats lacking testicular macrophages showed significantly increased (twofold on average) serum concentrations of luteinizing hormone at 5 and 10 days after treatment. Serum luteinizing hormone concentrations drop to control values at 15 days after treatment. Serum testosterone concentrations were increased significantly (twofold on average) at 5, 10 and 15 days after treatment. No significant changes were found for follicle-stimulating hormone serum concentrations, or for the weights of the testes and sex accessory organs. Testicular histology was unchanged, except for the absence of testicular macrophages in Cl2MDP-lp-treated animals. Rats treated with NaCl or Cl2MDP-lp were injected with 100 IU of human chorionic gonadotrophin and sacrificed 2 h later. Serum testosterone concentrations increased 8.6- and 3.5-fold in NaCl and Cl2MDP-lp-treated rats, respectively, in response to acute human chorionic gonadotrophin treatment. These results point out the relevance of testicular macrophages for the regulation of the pituitary-testicular axis.