APPLICATION OF CONFOCAL LASER-SCANNING MICROSCOPY TO THE OBSERVATION OF BONE-BIOPSY SPECIMENS

被引：20

作者：

KAZAMA, JJ ^{[1
]}

GEJYO, F ^{[1
]}

EJIRI, S ^{[1
]}

OKADA, M ^{[1
]}

EI, I ^{[1
]}

ARAKAWA, M ^{[1
]}

OZAWA, H ^{[1
]}

机构：

[1] NIIGATA UNIV, SCH DENT, NIIGATA 95021, JAPAN

来源：

BONE | 1993年 / 14卷 / 06期

关键词：

CONFOCAL LASER SCANNING MICROSCOPY (CLSM); BONE BIOPSY; METHYL METHACRYLATE (MMA); VILLANUEVA BONE STAIN; 3-DIMENSIONAL IMAGE; BONE HISTOMORPHOMETRY;

D O I：

10.1016/8756-3282(93)90319-6

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Iliac bone biopsy specimens from five patients with endstage renal disease were observed by confocal laser scanning microscopy. Images of affected bone specimens were accurately focused, whereas images viewed with conventional light microscopy from thick ground sections were obscure. Especially at high magnifications, fine structures of bone cells, otherwise blurred, were clearly observed with confocal scanning microscopy. From thin-cut sections, images satisfactory for pathological diagnosis were viewed with light microscopy even at high magnification. However, the sections tended to shrink vertically compared with the cross-sectional images of the blocks observed directly by confocal laser scanning microscopy. A three-dimensional image of bone tissue was also constructed from serial optical sections. Confocal laser scanning microscopy is a useful technique for observing bone tissues, and may become essential for the evaluation of bone biopsy specimens.

引用

页码：885 / 889

页数：5

共 20 条

[1]

[Anonymous], [No title captured], Patent No. [3013467 A, 3013467]

[2]

BOYDE A, 1986, Scanning Electron Microscopy, P1537

[3] 3-D ANALYSIS OF TETRACYCLINE FLUORESCENCE IN BONE BY TANDEM SCANNING REFLECTED LIGHT-MICROSCOPY [J].