A PURIFICATION METHOD FOR LABILE VARIANTS OF RIBONUCLEASE-T1

We present a new procedure for the rapid production of ribonuclease Ti variants with decreased stability which could not be purified in satisfying amounts by the existing methods. The major changes from the established procedures are the following. (i) The cells were grown at 28°C rather than at 37°C. (ii) The entire purification was performed at low temperatures (4°C). (iii) Materials for chromatography with high flow rates were used to accelerate protein isolation. (iv) The pH was lowered from 7.5 to 6.0, a condition under which RNase T1 is much more stable. The use of this improved procedure allowed the purification of the labile P39G and P73V variants of RNase T1. By the same technique 300 mg of the wild-type protein could be isolated from 10 liters liquid culture within 3 days. The P39G and the P73V mutations strongly decrease the stability of RNase T1 and the midpoints of the reversible thermal unfolding transition are lowered by 16 and 6°C, respectively, relative to that of the wild-type protein. The decrease in temperature during fermentation and the rapid purification at low temperature and under solvent conditions where the stability of the proteins is high are probably the major reasons for the dramatic increase in yield of these labile variants of RNase T1. Such an approach should be valuable for the production of recombinant proteins in general. © 1993 Academic Press. All rights reserved.