PERFORMANCE OF AN ETHOXYETHYLACRYLATE STATIONARY-PHASE FOR OPEN-TUBULAR LIQUID-CHROMATOGRAPHY

被引：29

作者：

SWART, R ^{[1
]}

KRAAK, JC ^{[1
]}

POPPE, H ^{[1
]}

机构：

[1] UNIV AMSTERDAM,ANALYT CHEM LAB,1018 WV AMSTERDAM,NETHERLANDS

来源：

JOURNAL OF CHROMATOGRAPHY A | 1995年 / 689卷 / 02期

关键词：

D O I：

10.1016/0021-9673(94)00887-F

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Fused-silica capillaries of I.D. ca 11.4 mu m were coated with polyacrylate layers. The layers consisted of copolymers of silicone acrylate and ethoxyethylacrylate which were polymerized in situ by UV irradiation. Capillaries with phase ratios up to 0.9 were prepared. This results in very high mass loadabilities so that solute amounts suitable for UV detection can be injected without overloading the stationary phase. This is illustrated by the separation of various mixtures containing phenolic and aromatic compounds. The retention behaviour and the kinetic performance were studied with both Wand laser-induced fluorescence detection. On a 5-m column more than 10(6) plates could be generated for anthracene. Further, the possibility of on-column preconcentration and the effect of temperature on the kinetic performance were investigated on this stationary phase.

引用

页码：177 / 187

页数：11

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