PLASMID-ENCODED DETERMINANTS FOR BACTERIOCIN PRODUCTION AND IMMUNITY IN A LACTOCOCCUS-LACTIS STRAIN AND PURIFICATION OF THE INHIBITORY PEPTIDE

被引：25

作者：

DUFOUR, A

THUAULT, D

BOULLIOU, A

BOURGEOIS, CM

LEPENNEC, JP

机构：

[1] UNIV RENNES 1, GENET MOLEC LAB, URA 256, CAMPUS BEAULIEU, F-35042 RENNES, FRANCE

[2] ASSOC DEV RECH APPL IND AGRICOLES & ALIMENTAIRES, F-29191 QUIMPER, FRANCE

来源：

JOURNAL OF GENERAL MICROBIOLOGY | 1991年 / 137卷

关键词：

D O I：

10.1099/00221287-137-10-2423

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Lactococcin, a bacteriocin produced by Lactococcus lactis subsp. lactis ADRIA 85LO30, was purified as a 2.3-2.4 kDa peptide. Six non-bacteriocin-producing (Bac-) and non-immune (Imm-) strains were isolated after curing experiments. These strains had in common the loss or modification of two plasmids: pOS4 (32 kb) and pOS5 (70 kb). By comparing pOS5 and several modified plasmids, a DNA region from pOS5 of about 10 kb, which was necessary for wild-type bacteriocin production and immunity, was identified.

引用

页码：2423 / 2429

页数：7

共 29 条

[1] GENETIC AND PHYSICAL CHARACTERIZATION OF RECOMBINANT PLASMIDS ASSOCIATED WITH CELL-AGGREGATION AND HIGH-FREQUENCY CONJUGAL TRANSFER IN STREPTOCOCCUS-LACTIS ML3 [J].