SEQUENCE OF THE S-GENE FROM A VIRULENT BRITISH FIELD ISOLATE OF TRANSMISSIBLE GASTROENTERITIS VIRUS

被引：24

作者：

BRITTON, P

PAGE, KW

机构：

[1] A.F.R.C., Institute for Animal Health, Compton Laboratory, Compton, Newbury

来源：

VIRUS RESEARCH | 1990年 / 18卷 / 01期

关键词：

TRANSMISSIBLE GASTROENTERITIS VIRUS; CORONAVIRUS; SPIKE; PORCINE; CDNA;

D O I：

10.1016/0168-1702(90)90090-X

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Subgenomic mRNA from a virulent field isolate of porcine transmissible gastroenteritis virus (TGEV), strain FS772/70, was used to produce cDNA. The cDNA from three overlapping clones was sequenced by the chain termination method and two open reading frames (ORFs) were identified. The largest ORF, 4350bp, encoded a polypeptide of 1449 amino acids of relative molecular mass (M(r)) 159811, which contained 33 potential N-linked glycosylation sites, a cysteine-rich region, and a potential transmembrane region. The C-terminal half of this ORF showed homology to the S proteins of four other coronaviruses. The C-terminal half of this ORF showed homology to the S proteins of four other coronaviruses. The other ORF consisted of the 3'-end of a gene with homology to the carboxyl terminus of the F2 subunit of infectious bronchitis virus (IBV) RNA polymerase.

引用

页码：71 / 80

页数：10

共 27 条

[11]

Delmas, Rasschaert, Godet, Gelfi, Laude, Four major antigenic sites of the coronavirus transmissible gastroenteritis virus are located on the amino-terminal half of spike glycoprotein S, J. Gen. Virol., 71, pp. 1313-1323, (1990)

[12]

Eisenberg, Schwarz, Komaromy, Wall, Analysis of membrane and surface protein sequences with the hydrophobic moment plot, J. Mol. Biol., 179, pp. 125-142, (1984)

[13]

Garwes, Lucas, Higgins, Pike, Cartwright, Antigenicity of structural components from porcine transmissible gastroenteritis virus, Vet. Microbiol., 3, pp. 179-190, (1978)

[14]

Garwes, Stewart, Elleman, Identification of epitopes of immunological importance on the peplomer of porcine transmissible gastroenteritis virus, Coronaviruses. Adv. Exp. Med. Biol., 218, pp. 509-515, (1987)

[15]

Garwes, Stewart, Britton, The polypeptide of M<sub>r</sub> 14000 of porcine transmissible gastroenteritis virus: gene assignment and intracellular location, J. Gen. Virol., 70, pp. 2495-2499, (1989)

[16]

Jacobs, de Groot, van der Zeijst, Horzinek, Spaan, The nucleotide sequence of the peplomer gene of porcine transmissible gastroenteritis virus (TGEV): comparison with the sequence of the peplomer protein of feline infectious peritonitis virus (FIPV), Virus Res., 8, pp. 363-371, (1987)

[17]

Jimenez, Correa, Melgosa, Bullido, Enjuanes, Critical epitopes in transmissible gastroenteritis virus neutralization, J. Virol., 60, pp. 131-139, (1986)

[18]

Laude, Chapsal, Gelfi, Labiau, Grosclaude, Antigenic structure of transmissible gastroenteritis virus. I. Properties of monoclonal antibodies directed against virion proteins, J. Gen. Virol., 67, pp. 119-130, (1986)

[19]

Parker, Yoo, Cox, Babiuk, Primary structure of the S peplomer gene of bovine coronavirus and surface expression in insect cells, J. Gen. Virol., 71, pp. 263-270, (1990)

[20]

Posthumus, Lenstra, Schaaper, van Nieuwstadt, Enjuanes, Meloen, Analysis and simulation of a neutralizing epitope of transmissible gastroenteritis virus, J. Virol., 64, pp. 3304-3309, (1990)

← 1 2 3 →