ESTABLISHMENT AND CHARACTERIZATION OF SYNOVIAL CELL CLONES WITH INTEGRATED HUMAN T-CELL LEUKEMIA-VIRUS TYPE-I

被引：17

作者：

HASUNUMA, T ^{[1
]}

NAKAJIMA, T ^{[1
]}

AONO, H ^{[1
]}

SATO, K ^{[1
]}

MATSUBARA, T ^{[1
]}

YAMAMOTO, K ^{[1
]}

NISHIOKA, K ^{[1
]}

机构：

[1] KOBE UNIV, DEPT ORTHOPED, KOBE 657, JAPAN

来源：

CLINICAL IMMUNOLOGY AND IMMUNOPATHOLOGY | 1994年 / 72卷 / 01期

关键词：

D O I：

10.1006/clin.1994.1111

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Human T cell leukemia virus type-I (HTLV-I)-integrated synovial cell clones (SCCs) were established from four HTLV-I-infected patients with chronic proliferative arthropathy. Extracted DNAs of these SCCs were examined for the existence of HTLV-I proviral DNA. Electron microscopic analysis and immunohistochemical staining were also performed to examine the nature of the HTLV-I integrated SCCs. SCCs derived from all four patients consisted of either HTLV-I integrated or nonintegrated cells. HTLV-I integrated SCCs also produced viral messenger RNA which was detected by reverse transcriptase-polymerase chain reaction as well as viral protein which was detected by immunohistochemical staining. Phenotypically, these SCCs containing HTLV-I proviral DNA were shown to be all cell types as observed by electron microscopic analysis and immunohistochemical analysis. Moreover, expression of HTLV-I glycoprotein was greater in SCCs with a monocyte marker. These findings suggest that SCCs harboring HTLV-I have an important role in synovial hyperplasia. (C) 1994 Academic Press, Inc.

引用

页码：90 / 97

页数：8

共 37 条

[21] OVERGROWTH OF HUMAN SYNOVIAL-CELLS DRIVEN BY THE HUMAN T-CELL LEUKEMIA-VIRUS TYPE-I TAX GENE [J].