CRYSTALLIZATION AND PRELIMINARY CRYSTALLOGRAPHIC STUDY OF NEURAMINIDASE FROM MICROMONOSPORA-VIRIDIFACIENS

被引:8
作者
TAYLOR, G
DINELEY, L
GLOWKA, M
LAVER, G
机构
[1] TECH UNIV LODZ,INST GEN CHEM,PL-90924 LODZ,POLAND
[2] AUSTRALIAN NATL UNIV,JOHN CURTIN MED SCH,CANBERRA,ACT 2601,AUSTRALIA
关键词
NEURAMINIDASE; SIALIDASE; CRYSTALLOGRAPHY; MICROMONOSPORA-VIRIDIFACIENS;
D O I
10.1016/0022-2836(92)90112-W
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Single crystals of neuraminidase from the bacterium Micromonospora viridifaciens were obtained using the hanging drop vapour diffusion method and polyethylene glycol as precipitant at pH 5.0 or 5.5. The crystals belong to the orthorhombic space group P212121, with unit cell dimensions a = 48.14 A ̊, b = 82.73 A ̊, c = 84.75 A ̊ and with one molecule in the asymmetric unit. Diffraction extends to at least 1.7 Å. © 1992.
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页码:1135 / 1136
页数:2
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