MINIATURIZATION OF 3 CARBOHYDRATE ANALYSES USING A MICROSAMPLE PLATE READER

被引：358

作者：

FOX, JD ^{[1
]}

ROBYT, JF ^{[1
]}

机构：

[1] IOWA STATE UNIV SCI & TECHNOL,DEPT BIOCHEM & BIOPHYS,AMES,IA 50011

来源：

ANALYTICAL BIOCHEMISTRY | 1991年 / 195卷 / 01期

关键词：

D O I：

10.1016/0003-2697(91)90300-I

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Three carbohydrate analyses (reducing value by copper-bicinchoninate, total carbohydrate by phenol-sulfuric acid, and d-glucose by glucose oxidase) have been miniaturized using a microsample plate reader. The use of the reducing-value procedure to measure the hydrolysis of starch by α-amylase and the use of the glucose oxidase method to measure the hydrolysis of lactose by lactase are illustrated. © 1991.

引用

页码：93 / 96

页数：4

共 10 条

[1] USE OF A 96-WELL MICROPLATE READER FOR MEASURING ROUTINE ENZYME-ACTIVITIES
ASHOUR, MBA
GEE, SJ
HAMMOCK, BD
[J]. ANALYTICAL BIOCHEMISTRY, 1987, 166 (02) : 353 - 360
[2] A SEMIAUTOMATED SYSTEM FOR MEASUREMENT OF 96 SIMULTANEOUS SPECTROPHOTOMETRIC ENZYME ASSAYS
BUTLER, EG
GILL, R
GARYCH, L
[J]. ANALYTICAL BIOCHEMISTRY, 1988, 170 (02) : 402 - 408
[3] COLORIMETRIC METHOD FOR DETERMINATION OF SUGARS AND RELATED SUBSTANCES
DUBOIS, M
GILLES, KA
HAMILTON, JK
REBERS, PA
SMITH, F
[J]. ANALYTICAL CHEMISTRY, 1956, 28 (03) : 350 - 356
[4] DETERMINATION OF GLUCOSE IN PRESENCE OF MALTOSE AND ISOMALTOSE BY A STABLE, SPECIFIC ENZYMIC REAGENT
FLEMING, ID
PEGLER, HF
[J]. ANALYST, 1963, 88 (105) : 967 - &
[5] ADAPTATION OF THE NELSON-SOMOGYI REDUCING-SUGAR ASSAY TO A MICROASSAY USING MICROTITER PLATES
GREEN, F
CLAUSEN, CA
HIGHLEY, TL
[J]. ANALYTICAL BIOCHEMISTRY, 1989, 182 (02) : 197 - 199
[6] STRUCTURE STUDIES OF AMYLOSE-V COMPLEXES AND RETROGRADED AMYLOSE BY ACTION OF ALPHA-AMYLASES, AND A NEW METHOD FOR PREPARING AMYLODEXTRINS
JANE, JL
ROBYT, JF
[J]. CARBOHYDRATE RESEARCH, 1984, 132 (01) : 105 - 118
[7] COLORIMETRIC DETERMINATION OF NEUTRAL SUGARS BY A RESORCINOL SULFURIC-ACID MICROMETHOD
MONSIGNY, M
PETIT, C
ROCHE, AC
[J]. ANALYTICAL BIOCHEMISTRY, 1988, 175 (02) : 525 - 530
[8] OXIDATION OF GLUCOSE + RELATED COMPOUNDS BY GLUCOSE OXIDASE FROM ASPERGILLUS NIGER
PAZUR, JH
KLEPPE, K
[J]. BIOCHEMISTRY, 1964, 3 (04) : 578 - &
[9] Stein EA, 1961, BIOCHEM PREP, V8, P34
[10] ASSAY OF REDUCING SUGARS IN THE NANOMOLE RANGE WITH 2,2'-BICINCHONINATE
WAFFENSCHMIDT, S
JAENICKE, L
[J]. ANALYTICAL BIOCHEMISTRY, 1987, 165 (02) : 337 - 340

← 1 →