CDNA CLONING OF HUMAN AND RAT-BRAIN MYOINOSITOL MONOPHOSPHATASE - EXPRESSION AND CHARACTERIZATION OF THE HUMAN RECOMBINANT ENZYME

被引:97
作者
MCALLISTER, G
WHITING, P
HAMMOND, EA
KNOWLES, MR
ATACK, JR
BAILEY, FJ
MAIGETTER, R
RAGAN, CI
机构
[1] Merck Sharp and Dohme Research Labs, Terlings Park, Harlow, Essex CM20 2QR, Eastwick Road
关键词
D O I
10.1042/bj2840749
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Inositol monophosphatase (EC 3.1.3.25) is a key enzyme in the phosphoinositide cell-signalling system. Its role is to provide inositol required for the resynthesis of phosphatidylinositol and polyphosphoinositides. It is the probable pharmacological target for lithium action in brain. Using probes derived from the bovine inositol monophosphatase cDNA we have isolated cDNA clones encoding the human and rat brain enzymes. The enzyme is highly conserved in all three species (79 % identical). The coding region of the human cDNA was inserted into a bacterial expression vector. The expressed recombinant enzyme was purified and its biochemical properties examined. The human enzyme is very similar to the bovine enzyme.
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收藏
页码:749 / 754
页数:6
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