EVALUATION OF NESTED POLYMERASE CHAIN METHODS FOR THE DETECTION OF HUMAN CORONAVIRUSES 229E AND OC43

被引：69

作者：

MYINT, S

JOHNSTON, S

SANDERSON, G

SIMPSON, H

机构：

[1] UNIV LEICESTER,DEPT CHILD HLTH,LEICESTER,LEICS,ENGLAND

[2] UNIV SOUTHAMPTON,DEPT MED,SOUTHAMPTON,HANTS,ENGLAND

来源：

MOLECULAR AND CELLULAR PROBES | 1994年 / 8卷 / 05期

关键词：

CORONAVIRUS; RESPIRATORY TRACT; DIAGNOSIS; POLYMERASE CHAIN REACTION;

D O I：

10.1006/mcpr.1994.1052

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Currently, the diagnosis of human respiratory coronavirus infection is either stow or insensitive. This paper describes nested polymerase chain reaction assays for the detection of human coronaviruses OC43 and 229E. The specificity and sensitivity of the assays have been determined and they have been applied to the detection of the viruses in nasal aspirates. These assays are more rapid and sensitive than cell culture and may replace the latter as the diagnostic method of choice.

引用

页码：357 / 364

页数：8

共 10 条

[1] USE OF THE POLYMERASE CHAIN-REACTION TO DETECT RHINOVIRUS IN WHEEZY INFANTS [J].