SCREENING FOR ALPHA-AMYLASE IN CEREALS - IMPROVED GEL-DIFFUSION ASSAY USING A DYE-LABELED STARCH SUBSTRATE

被引：17

作者：

HEJGAARD, J ^{[1
]}

GIBBONS, GC ^{[1
]}

机构：

[1] CARLSBERG RES CTR,DEPT BIOTECHNOL,DK-2500 COPENHAGEN,DENMARK

来源：

CARLSBERG RESEARCH COMMUNICATIONS | 1979年 / 44卷 / 01期

关键词：

barley; blue-starch substrate; Rapid α-amylase test; rye; triticale; wheat;

D O I：

10.1007/BF02910868

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Radial diffusion into a gel slab containing dye-labelled starch as substrate provides a specific, reliable and rapid α-amylase estimation of potential use in screening of a large number of cereal samples, including single grains or half-grains in early stages of a breeding program. The method has also been used to monitor α-amylase activity in column chromatography eluates. © 1979 Carlsberg Laboratory.

引用

页码：21 / 25

页数：5

共 11 条

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[7] GEL DIFFUSION ASSAY FOR DIASTATIC ACTIVITY, AND ITS USE IN PLANT BREEDING
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[J]. JOURNAL OF THE INSTITUTE OF BREWING, 1972, 78 (04) : 310 - &
[8] HEJGAARD J, 1979, HEREDITAS, V90, P145
[9] ELECTROPHORETIC STUDIES OF ALPHA-AMYLACE IN WHEAT .2
OLERED, R
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[J]. JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 1970, 21 (07) : 385 - &
[10] AFFINITY CHROMATOGRAPHY OF CEREAL ALPHA-AMYLASE
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HILL, RD
[J]. ANALYTICAL BIOCHEMISTRY, 1976, 73 (02) : 430 - 433

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