GENOMIC STRUCTURE OF AND A CARDIAC PROMOTER FOR THE MOUSE CARNITINE PALMITOYLTRANSFERASE-II GENE

被引:16
作者
GELB, BD [1 ]
机构
[1] MT SINAI SCH MED, DEPT HUMAN GENET, NEW YORK, NY 10029 USA
关键词
D O I
10.1016/S0888-7543(05)80368-4
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The mouse carnitine palmitoyltransferase II gene hasbeen isolated and its genomic structure determined. The gene contained five exons, including an unusually long 1305-nt exon 4. The exon/intron boundaries were sequenced and conformed to the consensus splice junction sequences. The transcription start site was determined from adult mouse heart mRNA by primer extension analysis and confirmed by 5′ rapid amplification of cDNA ends. A promoter that differs from the previously published promoter for the carnitine palmitoyltransferase II gene was isolated and sequenced. When placed in a mammalian expression vector, this promoter was shown to drive the chloramphenicol acetyltransferase gene in mouse hepatoma cells. This new promoter is the major driver of carnitine palmitoyltransferase II gene expression in heart and may also be active in liver. © 1993 Academic Press, Inc.
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页码:651 / 655
页数:5
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