GENOMIC STRUCTURE OF AND A CARDIAC PROMOTER FOR THE MOUSE CARNITINE PALMITOYLTRANSFERASE-II GENE

被引：16

作者：

GELB, BD ^{[1
]}

机构：

[1] MT SINAI SCH MED, DEPT HUMAN GENET, NEW YORK, NY 10029 USA

来源：

GENOMICS | 1993年 / 18卷 / 03期

关键词：

D O I：

10.1016/S0888-7543(05)80368-4

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The mouse carnitine palmitoyltransferase II gene hasbeen isolated and its genomic structure determined. The gene contained five exons, including an unusually long 1305-nt exon 4. The exon/intron boundaries were sequenced and conformed to the consensus splice junction sequences. The transcription start site was determined from adult mouse heart mRNA by primer extension analysis and confirmed by 5′ rapid amplification of cDNA ends. A promoter that differs from the previously published promoter for the carnitine palmitoyltransferase II gene was isolated and sequenced. When placed in a mammalian expression vector, this promoter was shown to drive the chloramphenicol acetyltransferase gene in mouse hepatoma cells. This new promoter is the major driver of carnitine palmitoyltransferase II gene expression in heart and may also be active in liver. © 1993 Academic Press, Inc.

引用

页码：651 / 655

页数：5

共 23 条

[1] PILOT SCALE PURIFICATION OF ALPHA-GALACTOSIDASE-A FROM COHN FRACTION-IV-1 OF HUMAN-PLASMA [J].

BISHOP, DF ;

WAMPLER, DE ;

SGOURIS, JT ;

BONEFELD, RJ ;

ANDERSON, DK ;

HAWLEY, MC ;

SWEELEY, CC .

BIOCHIMICA ET BIOPHYSICA ACTA, 1978, 524 (01) :109-120

[2] FLUOROMETRIC ASSAY OF PROTEINS IN NANOGRAM RANGE [J].

BOHLEN, P ;

STEIN, S ;

DAIRMAN, W ;

UDENFRIEND, S .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1973, 155 (01) :213-220

[3] ISOLATION AND CHARACTERIZATION OF THE PROMOTER FOR THE GENE CODING FOR THE 68 KDA CARNITINE PALMITOYLTRANSFERASE FROM THE RAT [J].

BRADY, PS ;

PARK, EA ;

LIU, JS ;

HANSON, RW ;

BRADY, LJ .

BIOCHEMICAL JOURNAL, 1992, 286 :779-783

[4]

BREATHNACH R, 1981, ANNU REV BIOCHEM, V50, P349, DOI 10.1146/annurev.bi.50.070181.002025

[5]

BROWN NF, 1991, J BIOL CHEM, V266, P15446

[6]

CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2

[7] INFANTILE FORM OF CARNITINE PALMITOYLTRANSFERASE-II DEFICIENCY WITH HEPATOMUSCULAR SYMPTOMS AND SUDDEN-DEATH - PHYSIOPATHOLOGICAL APPROACH TO CARNITINE PALMITOYLTRANSFERASE-II DEFICIENCIES [J].

DEMAUGRE, F ;

BONNEFONT, JP ;

COLONNA, M ;

CEPANEC, C ;

LEROUX, JP ;

SAUDUBRAY, JM .

JOURNAL OF CLINICAL INVESTIGATION, 1991, 87 (03) :859-864

[8] IMMUNOQUANTITATIVE ANALYSIS OF HUMAN CARNITINE PALMITOYLTRANSFERASE-I AND PALMITOYLTRANSFERASE-II DEFECTS [J].

DEMAUGRE, F ;

BONNEFONT, JP ;

CEPANEC, C ;

SCHOLTE, J ;

SAUDUBRAY, JM ;

LEROUX, JP .

PEDIATRIC RESEARCH, 1990, 27 (05) :497-500

[9] MUSCLE CARNITINE PALMITYLTRANSFERASE DEFICIENCY AND MYOGLOBINURIA [J].

DIMAURO, S ;

DIMAURO, PMM .

SCIENCE, 1973, 182 (4115) :929-931

[10] THE STRUCTURE AND EVOLUTION OF THE HUMAN BETA-GLOBIN GENE FAMILY [J].

EFSTRATIADIS, A ;

POSAKONY, JW ;

MANIATIS, T ;

LAWN, RM ;

OCONNELL, C ;

SPRITZ, RA ;

DERIEL, JK ;

FORGET, BG ;

WEISSMAN, SM ;

SLIGHTOM, JL ;

BLECHL, AE ;

SMITHIES, O ;

BARALLE, FE ;

SHOULDERS, CC ;

PROUDFOOT, NJ .

CELL, 1980, 21 (03) :653-668

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