PATTERNS OF PROTEIN-SYNTHESIS FOLLOWING HEAT-SHOCK IN PUPAE OF DROSOPHILA-MELANOGASTER

被引：22

作者：

CHOMYN, A ^{[1
]}

MOLLER, G ^{[1
]}

MITCHELL, HK ^{[1
]}

机构：

[1] CALTECH, DIV BIOL, PASADENA, CA 91125 USA

来源：

DEVELOPMENTAL GENETICS | 1979年 / 1卷 / 01期

关键词：

Drosophila melanogaster; heat shock; phenocopies; protein synthesis; pupae;

D O I：

10.1002/dvg.1020010109

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Pupae of Drosophila melanogaster were heat‐shocked under conditions required to induce phenocopies in more than 90% of the flies that subsequently emerge. The effects of these treatments on protein synthesis in two tissues (thoracic epithelium and brain) were followed for several hours after the heat treatments. Results from pulse‐labeling and protein separations on sodium dodecylsulfate (SDS) acrylamide gels showed a virtually complete cessation of protein synthesis immediately after the shock, followed by a noncoordinate resumption of the starting pattern. Similar experiments following double heat shocks demonstrated a more rapid resumption of synthesis of heat shock proteins after two successive heat treatments than after a single one. Copyright © 1979 Wiley‐Liss, Inc.

引用

页码：77 / 95

页数：19