THE 4-NITROQUINOLINE 1-OXIDE MUTATIONAL SPECTRUM IN SINGLE-STRANDED-DNA IS CHARACTERIZED BY GUANINE TO PYRIMIDINE TRANSVERSIONS

被引：40

作者：

FRONZA, G ^{[1
]}

CAMPOMENOSI, P ^{[1
]}

IANNONE, R ^{[1
]}

ABBONDANDOLO, A ^{[1
]}

机构：

[1] UNIV GENOA,CATTEDRA GENET,I-16126 GENOA,ITALY

来源：

NUCLEIC ACIDS RESEARCH | 1992年 / 20卷 / 06期

关键词：

D O I：

10.1093/nar/20.6.1283

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

4-Nitroquinoline-1-oxide is a potent mutagen and carcinogen which induces two main guanine adducts at positions C8 and N2. In ds or ss damaged DNA the ratio C8/N2 adducts is 1:2 and 8 - 10:1, respectively. In bacteria and yeast 4NQO has been shown to be a base substitution mutagen acting at G residues inducing mainly G to A transitions. We determined the mutational spectrum induced by the 4NQO metabolite, acetoxy-4-aminoquinoline 1-oxide, in the M13lacZ'/E.coli lacZ-DELTA-M15 alpha complementation assay using ssDNA. Among 68 Ac-4HAQO induced mutants, G to Pyr transversion was the most frequent base substitution observed. By comparison with dsDNA based systems, our data suggest that dGuo-C8-AQO induces G to Pyr transversions. A mechanism to explain how this lesion may induce transversions is proposed.

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页码：1283 / 1287

页数：5

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