STRUCTURAL ORGANIZATION OF THE HCTLA-1 GENE ENCODING HUMAN GRANZYME-B

被引：31

作者：

HADDAD, P

CLEMENT, MV

BERNARD, O

LARSEN, CJ

DEGOS, L

SASPORTES, M

MATHIEUMAHUL, D

机构：

[1] INST GENET MOLEC,INSERM,U301,27 RUE JULIETTE DODU,F-75010 PARIS,FRANCE

[2] HOP ST LOUIS,CTR HAYEM,INSERM,U93,F-75475 PARIS 10,FRANCE

来源：

GENE | 1990年 / 87卷 / 02期

关键词：

cloning; cytotoxicity; perforin; phage; λ; vector; plasmid; Recombinant DNA; serine esterase; T lymphocyte;

D O I：

10.1016/0378-1119(90)90311-E

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Cytotoxic T lymphocytes (CTLs) and natural killer/lymphokine-activated cells produce granzymes, a family of serine esterase proteins located in cytoplasmic granules. These might be involved in different cytotoxic pathways. We report the structural organization of the human gene encoding granzyme B (hCTLA-1). A 4.75-kb genomic DNA fragment containing all the sequences of granzyme B-encoding cDNA clones has been sequenced. The gene is composed of five exons and four introns. A comparison with the genomic organization of murine CCP1/CTLA-1 showed very similar structure and a 76% nucleotide homology in the coding sequences. This suggests that both genes may have a common ancestor. No typical regulatory element was detected in the 1160 bp upstream from the ATG start codon. The detection of a second locus related to hCTLA-1 is also described. © 1990.

引用

页码：265 / 271

页数：7

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