CRYSTALLIZATION OF THE B800-820 LIGHT-HARVESTING COMPLEX FROM RHODOPSEUDOMONAS-ACIDOPHILA STRAIN 7750

被引:14
作者
GUTHRIE, N
MACDERMOTT, G
COGDELL, RJ
FREER, AA
ISAACS, NW
HAWTHORNTHWAITE, AM
HALLOREN, E
LINDSAY, JG
机构
[1] UNIV GLASGOW,DEPT BOT,GLASGOW G12 8QQ,SCOTLAND
[2] UNIV GLASGOW,DEPT BIOCHEM,GLASGOW G12 8QQ,SCOTLAND
关键词
CRYSTALS; MEMBRANES; PROTEIN; LIGHT-HARVESTING; CRYSTALLOGRAPHY;
D O I
10.1016/0022-2836(92)91016-I
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The B800-820 light-harvesting complex, an integral membrane protein, from Rhodopseudomonas acidophila strain 7750 has been crystallized. The tabular plates have a hexagonal unit cell of a = b = 121·8 A ̊ and c = 283·1 A ̊ and belong to the space group R32. X-ray diffraction data have been collected to 6 Å resolution, using an area detector on a rotating anode source. The B800-820 light-harvesting complex is comprised of four low molecular weight apoproteins (B800-820α1, B800-820α2, B800-820β1 and B800-820β2). Polyacrylamide gel electrophoresis shows that the complex exists as an oligomeric assembly, with an apparent molecular weight of 92,000. © 1992.
引用
收藏
页码:527 / 528
页数:2
相关论文
共 13 条