CHARACTERIZATION OF THE TOBACCO EIF-4A GENE FAMILY

被引：38

作者：

OWTTRIM, GW

MANDEL, T

TRACHSEL, H

THOMAS, AAM

KUHLEMEIER, C

机构：

[1] UNIV BERN,INST PLANT PHYSIOL,CH-3013 BERN,SWITZERLAND

[2] UNIV BERN,INST BIOCHEM & MOLEC BIOL,CH-3013 BERN,SWITZERLAND

[3] UNIV UTRECHT,DEPT MOLEC & CELL BIOL,3584 CH UTRECHT,NETHERLANDS

来源：

PLANT MOLECULAR BIOLOGY | 1994年 / 26卷 / 06期

关键词：

CDNA GENE FAMILY; NEIF-4A; RNA HELICASE; TRANSLATION INITIATION FACTOR; NICOTIANA;

D O I：

10.1007/BF00019489

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Characterization of cDNAs encoding eukaryotic translation initiation factor 4A (eIF-4A) indicates the expression of a minimum of ten related genes in tobacco leaf cells. The ten groups fall into two gene families, NeIF-4A2 and NeIF-4A3. The majority of the cDNAs exhibit significant sequence similarity to the NeIF-4A2 family at both the DNA and deduced amino acid levels. Northern analysis using specific probes indicates variable expression of four family members in various tobacco organs. Western analysis, using an anti-tobacco eIF-4A polyclonal antibody, reveals a complex pattern of immunologically related polypeptides of approximately 46 kDa. Subcellular fractionation suggests that at least one eIF-4A-related polypeptide is located in the chloroplast where it is ribosome-associated.

引用

页码：1747 / 1757

页数：11

共 17 条

[1] DIFFERENTIAL EXPRESSION OF GENES ENCODING THE HYPUSINE-CONTAINING TRANSLATION INITIATION-FACTOR, ELF-5A, IN TOBACCO [J].