DIFFERENTIAL EXPRESSION OF GENES ENCODING THE HYPUSINE-CONTAINING TRANSLATION INITIATION-FACTOR, ELF-5A, IN TOBACCO

被引：51

作者：

CHAMOT, D ^{[1
]}

KUHLEMEIER, C ^{[1
]}

机构：

[1] UNIV BERN,INST PLANT PATHOL,ALTENBERGRAIN 21,CH-3013 BERN,SWITZERLAND

来源：

NUCLEIC ACIDS RESEARCH | 1992年 / 20卷 / 04期

基金：

加拿大自然科学与工程研究理事会;

关键词：

D O I：

10.1093/nar/20.4.665

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two Nicotiana plumbaginifolia cDNA clones, NeIF-5A1 and NeIF-5A2, encoding eukaryotic translation initiation factor eIF-5A (formerly called eIF-4D) were cloned by heterologous screening with Dictyostelium and human eIF-5A probes. eIF-5A is the only protein known to contain a unique amino acid modification, hypusine. Comparison of the Nicotiana deduced amino acid sequences with those of other eIF-5A polypeptides reveals conservation throughout the coding sequence, especially in the region of the hypusine residue. Transcript analysis reveals that NeIF-5A1 is preferentially expressed in photosynthetic tissues, while NeIF-5A2 is constitutively expressed in all plant tissues examined. A polyclonal antibody was raised against NeIF-5A1 overexpressed in E. coli. NeIF-5A1 antiserum crossreacts with an 18 kDa polypeptide doublet in all tobacco tissues examined. At least one polypeptide of ca. 18 kDa from a diversity of higher and lower plants crossreacts with NeIF-5A1 antiserum.

引用

页码：665 / 669

页数：5

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