PC10 MONOCLONAL-ANTIBODY TO PROLIFERATING CELL NUCLEAR ANTIGEN AS PROBE FOR CYCLING CELL DETECTION IN DEVELOPING-TISSUES - A COMBINED IMMUNOCYTOCHEMICAL AND FLOW CYTOMETRIC STUDY

被引：57

作者：

CASASCO, A

GIORDANO, M

DANOVA, M

CASASCO, M

CORNAGLIA, AI

CALLIGARO, A

机构：

[1] POLICLIN SAN MATTEO,IRCCS,SEZ CLIN MED 2,DIPARTIMENTO MED INTERNA & TERAPIA MED,I-27100 PAVIA,ITALY

[2] UNIV PAVIA,CTR GRANDI STRUMENTI,I-27100 PAVIA,ITALY

来源：

HISTOCHEMISTRY | 1993年 / 99卷 / 03期

关键词：

D O I：

10.1007/BF00269137

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Proliferating cell nuclear antigen (PCNA), also referred to as cyclin, is an auxiliary protein to DNA-polymerase delta and a proposed marker of replicating cells. We have investigated the applicability and limitations of PC10 monoclonal antibody to PCNA in a cell kinetics study of developing human and rat tissues by immunocytochemical and flow cytometric techniques. Our data demonstrate that the epitope recognized by PC10 antibody is resistant to wax embedding, but sensitive to aldehyde fixation; conversely, alcoholic fixative solutions preserve the immunoreactivity to PC10. Tissue distribution, DNA content and bromodeoxyuridine uptake confirm that PC10-immunoreactive cells in alcohol-fixed tissues are cycling (G1-, S- and G2-phases traversing) cells. It is concluded that the PC10 antibody can be regarded as a powerful tool to study cell kinetics and differentiation in developing tissues, provided that the tissue processing is adeguate.

引用

页码：191 / 199

页数：9