SEQUENCES OF GENES ENCODING NAPHTHALENE DIOXYGENASE IN PSEUDOMONAS-PUTIDA STRAINS G7 AND NCIB-9816-4

被引：262

作者：

SIMON, MJ

OSSLUND, TD

SAUNDERS, R

ENSLEY, BD

SUGGS, S

HARCOURT, A

SUEN, WC

CRUDEN, DL

GIBSON, DT

ZYLSTRA, GJ

机构：

[1] AMGEN INC,AMGEN CTR,THOUSAND OAKS,CA 91320

[2] UNIV IOWA,DEPT MICROBIOL,IOWA CITY,IA 52242

[3] UNIV IOWA,CTR BIOCATALYSIS & BIOPROCESSING,IOWA CITY,IA 52242

来源：

GENE | 1993年 / 127卷 / 01期

关键词：

D O I：

10.1016/0378-1119(93)90613-8

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The multicomponent enzyme, naphthalene dioxygenase, initiates the metabolism of naphthalene by Pseudomonas putida strains G7 (PpG7) and NCIB 9816-4 (Pp9816-4). The genes involved (nahAaAbAcAd) are encoded by the NAH7 and pDTG1 plasmids, respectively, and form part of the nah operon. The locations of the structural genes were determined on previously cloned fragments of DNA. The nucleotide (nt) sequences were determined for nahAaAb from Pp9816-4 and for nahAaAbAcAd from PpG7. The appropriate open reading frames were identified using N-terminal amino acid sequences determined from the purified proteins. The two nt sequences showed 93% homology, with the least homology seen upstream from the promoter region.

引用

页码：31 / 37

页数：7

共 24 条

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