PURIFICATION AND CHARACTERIZATION OF 2 DISTINCT LIPASES FROM CANDIDA-CYLINDRACEA

被引:207
作者
RUA, ML [1 ]
DIAZMAURINO, T [1 ]
FERNANDEZ, VM [1 ]
OTERO, C [1 ]
BALLESTEROS, A [1 ]
机构
[1] CSIC,INST QUIM FIS ROCASOLANO,MADRID 6,SPAIN
关键词
LIPASE; ISOENZYME; ENZYME PURIFICATION; ENZYME CHARACTERIZATION; CANDIDA-CYLINDRACEA; CANDIDA-RUGOSA;
D O I
10.1016/0304-4165(93)90134-T
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have purified and characterized two isoenzymes from a commercial lipase preparation of Candida cylindracea. The purification procedure includes ethanol precipitation and DEAE Sephacel and Sephacryl HR 100 chromatographies. Lipase A and lipase B were purified 11-fold with a 5% and 21% recovery in activity, respectively. The enzymes have similar amino acid content, N-terminal sequence and molecular weight, but differ on neutral sugar content, hydrophobicity, presence of isoforms and stability to pH and temperature. They also show some differences in the substrate specificity.
引用
收藏
页码:181 / 189
页数:9
相关论文
共 55 条
  • [1] CATALYTIC ACTIVITY AND ASSOCIATION OF PANCREATIC LIPASE
    ANTONOV, VK
    DYAKOV, VL
    MISHIN, AA
    ROTANOVA, TV
    [J]. BIOCHIMIE, 1988, 70 (09) : 1235 - 1244
  • [2] PURIFICATION AND SPECIFICITY OF LIPASES FROM GEOTRICHUM-CANDIDUM
    BAILLARGEON, MW
    [J]. LIPIDS, 1990, 25 (12) : 841 - 848
  • [3] POLYETHYLENE-GLYCOL MODIFICATION OF CANDIDA-RUGOSA LIPASE
    BAILLARGEON, MW
    SONNET, PE
    [J]. JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY, 1988, 65 (11) : 1812 - 1815
  • [4] REGIOSELECTIVE DEACYLATION OF 1,6-ANHYDRO-BETA-D-GALACTOPYRANOSE DERIVATIVES CATALYZED BY SOLUBLE AND IMMOBILIZED LIPASES
    BALLESTEROS, A
    BERNABE, M
    CRUZADO, C
    MARTINLOMAS, M
    OTERO, C
    [J]. TETRAHEDRON, 1989, 45 (22) : 7077 - 7082
  • [5] BEELEY JG, 1985, GLYCOPROTEIN PROTEOG, P351
  • [6] POSITIONAL AND CHAIN SPECIFICITIES OF CANDIDA-CYLINDRACEA LIPASE
    BENZONANA, G
    ESPOSITO, S
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1971, 231 (01) : 15 - +
  • [7] CONFORMATIONAL-CHANGES INDUCED IN LENS ALPHA-CRYSTALLINS AND GAMMA-CRYSTALLINS BY MODIFICATION WITH GLUCOSE-6-PHOSPHATE - IMPLICATIONS FOR CATARACT
    BESWICK, HT
    HARDING, JJ
    [J]. BIOCHEMICAL JOURNAL, 1987, 246 (03) : 761 - 769
  • [8] A SERINE PROTEASE TRIAD FORMS THE CATALYTIC CENTER OF A TRIACYLGLYCEROL LIPASE
    BRADY, L
    BRZOZOWSKI, AM
    DEREWENDA, ZS
    DODSON, E
    DODSON, G
    TOLLEY, S
    TURKENBURG, JP
    CHRISTIANSEN, L
    HUGEJENSEN, B
    NORSKOV, L
    THIM, L
    MENGE, U
    [J]. NATURE, 1990, 343 (6260) : 767 - 770
  • [9] THE ESTERASE PROFILE OF A LIPASE FROM CANDIDA-CYLINDRACEA
    BRAHIMIHORN, MC
    GUGLIELMINO, ML
    ELLING, L
    SPARROW, LG
    [J]. BIOCHIMICA ET BIOPHYSICA ACTA, 1990, 1042 (01) : 51 - 54
  • [10] BRAVO R, 1984, 2 DIMENSIONAL GEL EL, P4