ADSORPTION OF DNA TO MICA, SILYLATED MICE, AND MINERALS - CHARACTERIZATION BY ATOMIC-FORCE MICROSCOPY

被引：215

作者：

BEZANILLA, M

MANNE, S

LANEY, DE

LYUBCHENKO, YL

HANSMA, HG

机构：

[1] UNIV CALIF SANTA BARBARA,DEPT PHYS,SANTA BARBARA,CA 93106

[2] TECH UNIV MUNICH,DEPT PHYS,W-8000 MUNICH,GERMANY

[3] ARIZONA STATE UNIV,DEPT MICROBIOL,TEMPE,AZ 85287

[4] ARIZONA STATE UNIV,DEPT PHYS,TEMPE,AZ 85287

来源：

LANGMUIR | 1995年 / 11卷 / 02期

关键词：

D O I：

10.1021/la00002a050

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The nature of DNA adsorption to various substrates has been investigated by atomic force microscopy. The substrates used were mica, silylated mica, calcite, fluorite, and barite. The presence of a divalent cation, either in solution or accessible to the DNA on the substrate, greatly improves DNA adsorption. A comparison of DNA adsorption from different buffers onto mica and silylated mica shows that buffer composition is much less important for DNA adsorption to silylated mica and also suggests that for good adsorption DNA must be electrostatically adsorbed to the surface.

引用

页码：655 / 659

页数：5

共 25 条

[1] THE TWIST, WRITHE AND OVERALL SHAPE OF SUPERCOILED DNA CHANGE DURING COUNTERION-INDUCED TRANSITION FROM A LOOSELY TO A TIGHTLY INTERWOUND SUPERHELIX - POSSIBLE IMPLICATIONS FOR DNA-STRUCTURE IN-VIVO [J].