REPRODUCIBLE IMAGING AND DISSECTION OF PLASMID DNA UNDER LIQUID WITH THE ATOMIC FORCE MICROSCOPE

被引：484

作者：

HANSMA, HG

VESENKA, J

SIEGERIST, C

KELDERMAN, G

MORRETT, H

SINSHEIMER, RL

ELINGS, V

BUSTAMANTE, C

HANSMA, PK

机构：

[1] UNIV OREGON,INST MOLEC BIOL,EUGENE,OR 97403

[2] DIGITAL INSTRUMENTS INC,SANTA BARBARA,CA 93117

来源：

SCIENCE | 1992年 / 256卷 / 5060期

关键词：

D O I：

10.1126/science.256.5060.1180

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Reproducible images of uncoated DNA in the atomic force microscope (AFM) have been obtained by imaging plasmid DNA on mica in n-propanol. Specially sharpened AFM tips give images with reproducible features several nanometers in size along the DNA. Plasmids can be dissected in propanol by increasing the force applied by the AFM tip at selected locations.

引用

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页码：1180 / 1184

页数：5

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