SIMPLE AND SENSITIVE COLORIMETRIC METHOD FOR THE DETERMINATION OF LONG-CHAIN FREE FATTY-ACIDS IN SUBCELLULAR ORGANELLES

被引：78

作者：

NIXON, M ^{[1
]}

CHAN, SHP ^{[1
]}

机构：

[1] SYRACUSE UNIV,DEPT BIOL,BIOL RES LABS,SYRACUSE,NY 13210

来源：

ANALYTICAL BIOCHEMISTRY | 1979年 / 97卷 / 02期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0003-2697(79)90093-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A colorimetric assay for the determination of long-chain free fatty acids (FFA) is described. The FFA were extracted from subcellular organelles with chloroform:heptane:methanol. The copper soaps of FFA were determined colorimetrically with diphenylcarbazide. There are three advantages to employing the present modified procedure. (a) The sensitivity has been increased approximately twofold over that of the previous procedure of K. Falholt, B. Lund, and W. Falholt (1973, Clin. Chim. Acta46, 105-111); (b) it takes less time to complete the assay compared to the tedious procedures currently available; and (c) the presence of bovine serum albumin, a known FAA-binding protein, does not interfere with the assay procedure. The assay shows a linear response over the range of 10 to 130 nmol of FFA. The recovery of free fatty acids from mitochondria is 99%. © 1979.

引用

页码：403 / 409

页数：7

共 11 条

[1] ESTIMATION OF THE HIGHER FATTY ACIDS C7-C18 [J].

AYERS, CW .

ANALYTICA CHIMICA ACTA, 1956, 15 (01) :77-83

[2] PURIFICATION AND PROPERTIES OF ATPASE INHIBITOR FROM RAT-LIVER MITOCHONDRIA [J].

CHAN, SHP ;

BARBOUR, RL .

BIOCHIMICA ET BIOPHYSICA ACTA, 1976, 430 (03) :426-433

[3] COLORIMETRIC MICRO-DETERMINATION OF LONG-CHAIN FATTY ACIDS [J].

DUNCOMBE, WG .

BIOCHEMICAL JOURNAL, 1963, 88 (01) :7-&

[4] EASY COLORIMETRIC MICROMETHOD FOR ROUTINE DETERMINATION OF FREE FATTY-ACIDS IN PLASMA [J].

FALHOLT, K ;

LUND, B ;

FALHOLT, W .

CLINICA CHIMICA ACTA, 1973, 46 (02) :105-111

[5] COLORIMETRIC MICRO-DETERMINATION OF FREE FATTY ACIDS IN PLASMA [J].

LAURELL, S ;

TIBBLING, G .

CLINICA CHIMICA ACTA, 1967, 16 (01) :57-&

[6] COLORIMETRIC DETERMINATION OF FREE FATTY ACIDS [J].

LAUWERYS, RR .

ANALYTICAL BIOCHEMISTRY, 1969, 32 (02) :331-&

[7]

lwayama V, 1959, J PHARM SOC JAPAN, V79, P552

[8]

NOVAK M, 1965, J LIPID RES, V6, P431

[9] SPECIFIC DETERMINATION OF FREE FATTY ACID IN PLASMA [J].

REGOUW, BJM ;

CORNELIS.PJ ;

HELDER, RAP ;

SPIJKERS, JB ;

WEEBER, YMM .

CLINICA CHIMICA ACTA, 1971, 31 (01) :187-&

[10] NEW SALTING-OUT TECHNIQUE FOR COLORIMETRIC FREE FATTY-ACID ASSAYS [J].

SMITH, SW .

ANALYTICAL BIOCHEMISTRY, 1975, 67 (02) :531-539

← 1 2 →