ACTIVATION OF PROTEIN-KINASE-C BY PHORBOL ESTERS AND ARACHIDONIC-ACID REQUIRED FOR THE OPTIMAL POTENTIATION OF GLUTAMATE EXOCYTOSIS

被引:35
作者
HERRERO, I [1 ]
MIRASPORTUGAL, MT [1 ]
SANCHEZPRIETO, J [1 ]
机构
[1] UNIV COMPLUTENSE MADRID, FAC VET, DEPT BIOQUIM, E-28040 MADRID, SPAIN
关键词
SYNAPTOSOMES; GLUTAMATE EXOCYTOSIS; ARACHIDONIC ACID; PHORBOL ESTERS; PROTEIN KINASE-C; CALCIUM; MEMBRANE POTENTIAL;
D O I
10.1111/j.1471-4159.1992.tb08478.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The effects of arachidonic acid and phorbol esters in the Ca2+-dependent release of glutamate evoked by 4-aminopyridine (4-AP) in rat cerebrocortical synaptosomes were studied. In the absence of arachidonic acid, high concentrations (500 nM) of 4-beta-phorbol dibutyrate (4-beta-PDBu) were required to enhance the release of glutamate. However, in the presence of arachidonic acid, low concentrations of 4-beta-PDBu (1-50 nM) were effective in potentiating glutamate exocytosis. This potentiation of glutamate release by phorbol esters was not observed with the methyl ester of arachidonic acid, which does not activate protein kinase C. Moreover, pretreatment of synaptosomes with the protein kinase inhibitor staurosporine also prevented the stimulatory effect by arachidonic acid and phorbol esters. These results suggest that the activation of protein kinase C by both arachidonic acid and phorbol esters may play a role in the potentiation of glutamate exocytosis.
引用
收藏
页码:1574 / 1577
页数:4
相关论文
共 28 条
[11]   TRANSMITTER GLUTAMATE RELEASE FROM ISOLATED NERVE-TERMINALS - EVIDENCE FOR BIPHASIC RELEASE AND TRIGGERING BY LOCALIZED CA-2+ [J].
MCMAHON, HT ;
NICHOLLS, DG .
JOURNAL OF NEUROCHEMISTRY, 1991, 56 (01) :86-94
[12]  
MURAKAMI K, 1986, J BIOL CHEM, V261, P5424
[13]   DIRECT ACTIVATION OF PURIFIED PROTEIN KINASE-C BY UNSATURATED FATTY-ACIDS (OLEATE AND ARACHIDONATE) IN THE ABSENCE OF PHOSPHOLIPIDS AND CA-2+ [J].
MURAKAMI, K ;
ROUTTENBERG, A .
FEBS LETTERS, 1985, 192 (02) :189-193
[14]   CALCIUM-DEPENDENT AND CALCIUM-INDEPENDENT RELEASE OF GLUTAMATE FROM SYNAPTOSOMES MONITORED BY CONTINUOUS FLUOROMETRY [J].
NICHOLLS, DG ;
SIHRA, TS ;
SANCHEZPRIETO, J .
JOURNAL OF NEUROCHEMISTRY, 1987, 49 (01) :50-57
[16]   PHORBOL ESTER ENHANCEMENT OF NEUROTRANSMITTER RELEASE FROM RAT-BRAIN SYNAPTOSOMES [J].
NICHOLS, RA ;
HAYCOCK, JW ;
WANG, JKT ;
GREENGARD, P .
JOURNAL OF NEUROCHEMISTRY, 1987, 48 (02) :615-621
[17]   THE MOLECULAR HETEROGENEITY OF PROTEIN KINASE-C AND ITS IMPLICATIONS FOR CELLULAR-REGULATION [J].
NISHIZUKA, Y .
NATURE, 1988, 334 (6184) :661-665
[18]   SYNAPTOSOMAL PROTEIN-KINASE-C SUBSPECIES .B. DOWN-REGULATION PROMOTED BY PHORBOL ESTER AND ITS EFFECT ON EVOKED NOREPINEPHRINE RELEASE [J].
ODA, T ;
SHEARMAN, MS ;
NISHIZUKA, Y .
JOURNAL OF NEUROCHEMISTRY, 1991, 56 (04) :1263-1269
[19]   CA-2+-INDEPENDENT RELEASE OF GLUTAMATE DURING INVITRO ANOXIA IN ISOLATED NERVE-TERMINALS [J].
RUBIO, I ;
TORRES, M ;
MIRASPORTUGAL, MT ;
SANCHEZPRIETO, J .
JOURNAL OF NEUROCHEMISTRY, 1991, 57 (04) :1159-1164
[20]   SYNAPTOSOMAL PROTEIN-KINASE-C SUBSPECIES .A. DYNAMIC CHANGES IN THE HIPPOCAMPUS AND CEREBELLAR CORTEX CONCOMITANT WITH SYNAPTOGENESIS [J].
SHEARMAN, MS ;
SHINOMURA, T ;
ODA, T ;
NISHIZUKA, Y .
JOURNAL OF NEUROCHEMISTRY, 1991, 56 (04) :1255-1262